A young root-specific gene (ArMY2) from horseradish encoding a MYR II myrosinase with kinetic preference for the root-specific glucosinolate gluconasturtiin (Englisch)

In: Phytochemistry   ;  99 ;  26-35  ;  2013
  • ISSN:
  • Aufsatz (Zeitschrift)  /  Elektronische Ressource

Wie erhalte ich diesen Titel?


Download ist DRM geschützt.

Kommerziell Vergütung an den Verlag: 36,00 € Grundgebühr: 4,00 € Gesamtpreis: 40,00 €
Akademisch Vergütung an den Verlag: 36,00 € Grundgebühr: 2,00 € Gesamtpreis: 38,00 €

Graphical abstract A full-length cDNA (ArMY2) encoding an active myrosinase termed ArMy2 was isolated from Armoracia rusticana and heterologously expressed in Pichia pastoris. ArMY2 was specifically expressed in young roots. Display Omitted

Highlights ArMY2 is the first MYR II myrosinase gene described in Armoracia rusticana. ArMY2 encodes a myrosinase and is selectively expressed in young horseradish roots. Gluconasturtiin is the dominant glucosinolate in young horseradish roots. Recombinant ArMy2 produced in Pichia pastoris prefers gluconasturtiin as substrate. A specialized glucosinolate-myrosinase defense system may be active in young roots.

Abstract The pungent taste of horseradish is caused by isothiocyanates which are released from glucosinolates by myrosinases. These enzymes are encoded by genes belonging to one of two subfamilies, termed MYR I and MYR II, respectively. A MYR II-type myrosinase gene was identified for the first time in horseradish. The gene termed ArMY2 was only expressed in young roots. A full-length cDNA encoding a myrosinase termed ArMy2 was isolated and heterologously expressed in Pichia pastoris. The recombinant His-tagged enzyme was characterized biochemically. Substrate affinity was 5 times higher towards gluconasturtiin than towards sinigrin. Gluconasturtiin was found to be the most abundant glucosinolate in young horseradish roots while sinigrin dominated in storage roots and leaves. This indicates that a specialized glucosinolate–myrosinase defense system might be active in young roots.

Inhaltsverzeichnis – Band 99

Zeige alle Jahrgänge und Ausgaben

Die Inhaltsverzeichnisse werden automatisch erzeugt und basieren auf den im Index des TIB-Portals verfügbaren Nachweisen der enthaltenen Aufsätze. Die Anzeige der Jahrgänge kann aufgrund fehlender Aufsatznachweise unvollständig oder lückenhaft sein, obwohl die Zeitschrift komplett in der TIB verfügbar ist.

Graphical Contents List
| 2014
CYP94B3 activity against jasmonic acid amino acid conjugates and the elucidation of 12-O-β-glucopyranosyl-jasmonoyl-l-isoleucine as an additional metabolite
Kitaoka, Naoki / Kawaide, Hiroshi / Amano, Naruki / Matsubara, Takuya / Nabeta, Kensuke / Takahashi, Kosaku / Matsuura, Hideyuki | 2013
High level protein-purification allows the unambiguous polypeptide determination of latent isoform PPO4 of mushroom tyrosinase
Mauracher, Stephan G. / Molitor, Christian / Michael, Claudia / Kragl, Martin / Rizzi, Andreas / Rompel, Annette | 2013
A young root-specific gene (ArMY2) from horseradish encoding a MYR II myrosinase with kinetic preference for the root-specific glucosinolate gluconasturtiin
Loebers, Andreas / Müller-Uri, Frieder / Kreis, Wolfgang | 2013
Identification and characterization of omega-amidase as an enzyme metabolically linked to asparagine transamination in Arabidopsis
Zhang, Qianyi / Marsolais, Frédéric | 2013
Identification of UGT84A13 as a candidate enzyme for the first committed step of gallotannin biosynthesis in pedunculate oak (Quercus robur)
Mittasch, Juliane / Böttcher, Christoph / Frolova, Nadezhda / Bönn, Markus / Milkowski, Carsten | 2013
Detection of an O-methyltransferase synthesising acetosyringone in methyl jasmonate-treated tobacco cell-suspensions cultures
Negrel, Jonathan / Javelle, Francine / Wipf, Daniel | 2013
Metabolomics in melon: A new opportunity for aroma analysis
Allwood, J. William / Cheung, William / Xu, Yun / Mumm, Roland / De Vos, Ric C.H. / Deborde, Catherine / Biais, Benoit / Maucourt, Mickael / Berger, Yosef / Schaffer, Arthur A. et al. | 2013
Metabolic flux phenotype of tobacco hairy roots engineered for increased geraniol production
Masakapalli, Shyam K. / Ritala, Anneli / Dong, Lemeng / van der Krol, Alexander R. / Oksman-Caldentey, Kirsi-Marja / Ratcliffe, R. George / Sweetlove, Lee J. | 2013
Chemical variation in Jacobaea vulgaris is influenced by the interaction of season and vegetation successional stage
Carvalho, Sabrina / Macel, Mirka / Mulder, Patrick P.J. / Skidmore, Andrew / van der Putten, Wim H. | 2013
Evolution of alkaloid biosynthesis in the genus Narcissus
Berkov, Strahil / Martínez-Francés, Vanessa / Bastida, Jaume / Codina, Carles / Ríos, Segundo | 2013
Synthesis and antiviral effects of isosteviol-derived analogues against the hepatitis B virus
Huang, Tsurng-Juhn / Chou, Bo-Hon / Lin, Cheng-Wen / Weng, Jen-Hsien / Chou, Chang-Hung / Yang, Li-Ming / Lin, Shwu-Jiuan | 2013
Flavonoid and phenolic compounds from Salvia palaestina L. growing wild in Jordan and their antioxidant activities
Al-Qudah, Mahmoud A. / Al-Jaber, Hala I. / Abu Zarga, Musa H. / Abu Orabi, Sultan T. | 2013
Terpenoids from the roots of Drypetes hoaensis and their cytotoxic activities
Wittayalai, Sawangjitt / Mahidol, Chulabhorn / Prachyawarakorn, Vilailak / Prawat, Hunsa / Ruchirawat, Somsak | 2013
Phytochemical analysis of Rosa hybrida cv. ‘Jardin de Granville’ by HPTLC, HPLC-DAD and HPLC-ESI-HRMS: Polyphenolic fingerprints of six plant organs
Riffault, Ludivine / Destandau, Emilie / Pasquier, Laure / André, Patrice / Elfakir, Claire | 2013
Corrigendum to “Glycosylceramides from marine green microalga Tetraselmis sp.” [Phytochemistry 85 (2013) 107–114]
Arakaki, Atsushi / Iwama, Daisuke / Liang, Yue / Murakami, Nagisa / Ishikura, Masaharu / Tanaka, Tsuyoshi / Matsunaga, Tadashi | 2013
Announcement for the Phytochemical Society of North America (PSNA)
| 2014
Editorial Board/Publication Information
| 2014