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The torsion of the multi-span aqueduct body was studied under the lateral seismic excitation by FSI computational analysis. The results show that the difference of stiffness of both sides has little influence on the torque of the aqueduct body. However, the influence of the torque on both end sections of the aqueduct body is not to be neglected. For the simply-supported beam aqueduct, if the centre moment of the aqueduct is adopted as the controlling condition to design the whole aqueduct body and when the bending-torsion effect is small, the safety of anti-seismic structures can meet the requirements. Otherwise, on the section between the centre section of the aqueduct body and the end slot section, the safety of anti-seismic structures could not meet the requirements.