Heat shock augments myosin phosphatase target-subunit phosphorylation (English)

Biochemical and Biophysical Research Communications
Elsevier Inc. ; 2007

AbstractOur previous study demonstrated that heat shock augmented vascular contraction. In the present study, we hypothesized that heat shock augments myosin phosphatase target-subunit (MYPT1) phosphorylation resulting in augmented vascular contraction. Endothelium-denuded rat aortic rings were mounted in organ baths, exposed to heat shock (42°C for 45min), and subjected to contraction 4h after the heat shock followed by Western blot analysis for MLC20 (the 20kDa light chains of myosin II) or MYPT1. The contractile responses in both control and heat shock-treated aorta were inhibited by Y27632, an inhibitor of Rho-kinase. The level of the MLC20 and MYPT1Thr855 phosphorylation in response to KCl was higher in heat shock-treated aorta than that in timed-control. The increased MYPT1Thr855 phosphorylation was inhibited by Y27632 (1.0μM) in parallel with inhibition of MLC20 phosphorylation and vascular contraction. These results indicate that heat shock augments MYPT1 phosphorylation resulting in augmented vascular contraction.

How to get this document?
Download

Download is DRM protected.


Commercial Copyright fee: €36.00 Basic fee: €4.00 Total price: €40.00
Academic Copyright fee: €36.00 Basic fee: €2.00 Total price: €38.00

Document information


Table of contents – Volume 356, Issue 3

Show all volumes and issues

Tables of content are generated automatically and are based on records of articles contained that are available in the TIB-Portal index. Due to missing records of articles, the volume display may be incomplete, even though the whole journal is available at TIB.

523
Physical and functional interactions of human papillomavirus E2 protein with nuclear receptor coactivators
Wu, Meng-Hsun / Huang, Chi-Jung / Liu, Shu-Ting / Liu, Pei-Yao / Ho, Ching-Liang / Huang, Shih-Ming | 2007
529
Apoptosis induction by avian reovirus through p53 and mitochondria-mediated pathway
Chulu, Julius L.C. / Lee, Long H. / Lee, Ya C. / Liao, Shu H. / Lin, Feng L. / Shih, Wen L. / Liu, Hung J. | 2007
536
E74-like factor 2 regulates valosin-containing protein expression
Zhang, Binglin / Tomita, Yasuhiko / Qiu, Ying / He, Juxiang / Morii, Eiichi / Noguchi, Shinzaburo / Aozasa, Katsuyuki | 2007
542
Overexpression of membrane sialic acid-specific sialidase Neu3 inhibits matrix metalloproteinase-9 expression in vascular smooth muscle cells
Moon, Sung-Kwon / Cho, Seung-Hak / Kim, Kyung-Woon / Jeon, Jae Heung / Ko, Jeong-Heon / Kim, Bo Yeon / Kim, Cheorl-Ho | 2007
548
Oligomeric α-synuclein inhibits tubulin polymerization
Chen, Leo / Jin, Jinghua / Davis, Jeanne / Zhou, Yong / Wang, Yan / Liu, Jun / Lockhart, Paul J. / Zhang, Jing | 2007
548
Oligomeric a-synuclein inhibits tubulin polymerization
Chen, L. / Jin, J. / Davis, J. / Zhou, Y. / Wang, Y. / Liu, J. / Lockhart, P. J. / Zhang, J. | 2007
554
12S-lipoxygenase protein associates with α-actin fibers in human umbilical artery vascular smooth muscle cells
Weisinger, Gary / Limor, Rona / Marcus-Perlman, Yonit / Knoll, Esther / Kohen, Fortune / Schinder, Vera / Firer, Michael / Stern, Naftali | 2007
554
12S-lipoxygenase protein associates with a-actin fibers in human umbilical artery vascular smooth muscle cells
Weisinger, G. / Limor, R. / Marcus-Perlman, Y. / Knoll, E. / Kohen, F. / Schinder, V. / Firer, M. / Stern, N. | 2007
561
Spontaneous expression of neural phenotype and NGF, TrkA, TrkB genes in marrow stromal cells
Li, Na / Yang, Hui / Lu, Lingling / Duan, Chunli / Zhao, Chunli / Zhao, Huanying | 2007
569
Molecular modeling of the complex between Torpedo acetylcholine receptor and anti-MIR Fab198
Konstantakaki, Maria / Tzartos, Socrates J. / Poulas, Konstantinos / Eliopoulos, Elias | 2007
576
Identification and characterization of a novel spermatogenesis related gene LM23 in rat testis
Liu, Mei-ling / Pei, Kai-yan / Shi, Xin-quan / Liu, De-yu / Jia, Meng-chun | 2007
582
Effects of PKC on inhibition of delayed rectifier potassium currents by N/OFQ
Qu, Lihui / Li, Yurong / Tian, Hua / Wang, Zhigang / Cui, Lanwei / Jin, Hongbo / Wang, Wei / Yang, Lei | 2007
587
Glucose responsive insulin production from human embryonic germ (EG) cell derivatives
Clark, Gregory O. / Yochem, Robert L. / Axelman, Joyce / Sheets, Timothy P. / Kaczorowski, David J. / Shamblott, Michael J. | 2007
594
Identification of phostensin, a PP1 F-actin cytoskeleton targeting subunit
Kao, Shu-Chen / Chen, Chun-Yu / Wang, Shiu-Lan / Yang, Jaw-Ji / Hung, Wei-Chuan / Chen, Yi-Chen / Lai, Ning-Sheng / Liu, Hsin-Tzu / Huang, Hsien-lu / Chen, Hong-Chen et al. | 2007
599
The short chain fatty acid, butyrate, stimulates MUC2 mucin production in the human colon cancer cell line, LS174T
Hatayama, Hajime / Iwashita, Jun / Kuwajima, Akiko / Abe, Tatsuya | 2007
604
Three UDP-hexose 4-epimerases with overlapping substrate specificity coexist in E. coli O86:B7
Guo, Hongjie / Yi, Wen / Li, Lei / Wang, Peng George | 2007
610
Transgenic expression of osteoactivin in the liver attenuates hepatic fibrosis in rats
Abe, Hiroo / Uto, Hirofumi / Takami, Yoichiro / Takahama, Yuka / Hasuike, Satoru / Kodama, Mayumi / Nagata, Kenji / Moriuchi, Akihiro / Numata, Masatsugu / Ido, Akio et al. | 2007
616
Crystal structure study on human S100A13 at 2.0A resolution
Li, M. / Zhang, P. F. / Pan, X. W. / Chang, W. R. | 2007
616
Crystal structure study on human S100A13 at 2.0Å resolution
Li, Mei / Zhang, Ping-Feng / Pan, Xiao-Wei / Chang, Wen-Rui | 2007
622
Cloning and expression of human islet amyloid polypeptide in cultured cells
Bhattacharya, Susinjan / Naveena Lavanya Latha, J. / Kumresan, R. / Singh, Shashi | 2007
629
Role of SGK1 kinase in regulating glucose transport via glucose transporter GLUT4
Jeyaraj, Sankarganesh / Boehmer, Christoph / Lang, Florian / Palmada, Monica | 2007
636
Secretion of the glucose-regulated selenoprotein SEPS1 from hepatoma cells
Gao, Yuan / Pagnon, Joanne / Feng, Helen C. / Konstantopolous, Nicky / Jowett, Jeremy B.M. / Walder, Ken / Collier, Greg R. | 2007
642
Two transmembrane Cys residues are involved in 5-HT4 receptor dimerization
Berthouze, Magali / Rivail, Lucie / Lucas, Alexandre / Ayoub, Mohammed A. / Russo, Olivier / Sicsic, Sames / Fischmeister, Rodolphe / Berque-Bestel, Isabelle / Jockers, Ralf / Lezoualc’h, Frank | 2007
648
GRB10 binds to LRP6, the Wnt co-receptor and inhibits canonical Wnt signaling pathway
Tezuka, Norio / Brown, Anthony M.C. / Yanagawa, Shin-ichi | 2007
655
Truncated pStat5B is associated with the Idd4 locus in NOD mice
Davoodi-Semiromi, Abdoreza / McDuffie, Marcia / Litherland, Sally / Clare-Salzler, Michael | 2007
662
Preadipocyte response and impairment of differentiation in an inflammatory environment
Poulain-Godefroy, Odile / Froguel, Philippe | 2007
668
Phosphorylation of threonine 204 of DEAD-box RNA helicase DDX3 by cyclin B/cdc2 in vitro
Sekiguchi, Takeshi / Kurihara, Yoshiko / Fukumura, Junko | 2007
674
Plasma annexin A5 level relates inversely to the severity of coronary stenosis
van Tits, Lambertus J.H. / van Heerde, Waander L. / van der Vleuten, Gerly M. / de Graaf, Jacqueline / Grobbee, Diederick E. / van de Vijver, Lucy P.L. / Stalenhoef, Anton F. / Princen, Hans M. | 2007
681
OxLDL-induced gene expression patterns in CASMC are mimicked in apoE−/− mice aortas
Reeve, Janice L.V. / Stenson-Cox, Catherine / O’Doherty, Aideen / Pörn-Ares, Isabella / Ares, Mikko / O’Brien, Timothy / Samali, Afshin | 2007
687
Calcium-dependent conformational changes in guanylate cyclase-activating protein 2 monitored by cysteine accessibility
Helten, Andreas / Koch, Karl-Wilhelm | 2007
693
N-terminal hamartin-binding and C-terminal GAP domain of tuberin can separate in vivo
Momose, Shuji / Kobayashi, Toshiyuki / Tada, Norihiro / Itoyama, Shinji / Hino, Okio | 2007
699
Unscheduled overexpression of human WAPL promotes chromosomal instability
Ohbayashi, Tetsuya / Oikawa, Kosuke / Yamada, Kazuhiko / Nishida-Umehara, Chizuko / Matsuda, Yoichi / Satoh, Hitoshi / Mukai, Hiroyuki / Mukai, Kiyoshi / Kuroda, Masahiko | 2007
705
Influence of cholesterol and ergosterol on membrane dynamics using different fluorescent reporter probes
Shrivastava, Sandeep / Chattopadhyay, Amitabha | 2007
711
Involvement of up-regulation of PUMA in non-steroidal anti-inflammatory drug-induced apoptosis
Ishihara, Tomoaki / Hoshino, Tatsuya / Namba, Takushi / Tanaka, Ken-ichiro / Mizushima, Tohru | 2007
718
Heat shock augments myosin phosphatase target-subunit phosphorylation
Kim, Jee In / Jeon, Su Bun / Baek, Inji / Seok, Young Mi / Shin, Heung-Mook / Kim, In Kyeom | 2007
723
Transforming activity of purinergic receptor P2Y, G-protein coupled, 2 revealed by retroviral expression screening
Hatanaka, Hisashi / Takada, Shuji / Choi, Young Lim / Fujiwara, Shin-ichiro / Soda, Manabu / Enomoto, Munehiro / Kurashina, Kentaro / Watanabe, Hideki / Yamashita, Yoshihiro / Sugano, Kentaro et al. | 2007
727
STAT5 transcriptional activity is impaired by LIF in a mammary epithelial cell line
Rodriguez Granillo, Agustina / Boffi, Juan Carlos / Barañao, Lino / Kordon, Edith / Pecci, Adali / Guberman, Alejandra | 2007
733
Reversal of cocaine sensitization-induced behavioral sensitization normalizes GAD67 and GABAA receptor a2 subunit expression, and PKC z activity
Chen, Q. / Lee, T. H. / Wetsel, W. C. / Sun, Q. A. / Liu, Y. / Davidson, C. / Xiong, X. / Ellinwood, E. H. / Zhang, X. | 2007
733
Reversal of cocaine sensitization-induced behavioral sensitization normalizes GAD67 and GABAA receptor α2 subunit expression, and PKC ζ activity
Chen, Qiang / Lee, Tong H. / Wetsel, William C. / Sun, Qi-An / Liu, Yingmiao / Davidson, Colin / Xiong, Xueying / Ellinwood, Everett H. / Zhang, Xiuwu | 2007
739
Estimation of the embryotoxic effect of CBZ using an ES cell differentiation system
Murabe, Mayu / Yamauchi, Junji / Fujiwara, Yoko / Miyamoto, Yuki / Hiroyama, Masami / Sanbe, Atsushi / Tanoue, Akito | 2007
745
Intrinsic pro-angiogenic status of cystic fibrosis airway epithelial cells
Verhaeghe, Catherine / Tabruyn, Sebastien P. / Oury, Cecile / Bours, Vincent / Griffioen, Arjan W. | 2007
750
Construction and characterization of a bifunctional enzyme with deoxyribonuclease I and thioredoxin-like activities
Chen, Wei-Jung / Lo, Ting / Lai, Yu-Shen / Huang, Po-Tsang / Lin, Chai-Ching / Liao, Ta-Hsiu | 2007
756
Effect of glucolipotoxicity and rosiglitazone upon insulin secretion
Marshall, Catriona / Hitman, Graham A. / Cassell, Paul G. / Turner, Mark D. | 2007
763
Up-regulated expression of β3 integrin induced by dengue virus serotype 2 infection associated with virus entry into human dermal microvascular endothelial cells
Zhang, Jun-lei / Wang, Jia-li / Gao, Na / Chen, Zong-tao / Tian, Yan-ping / An, Jing | 2007
763
Up-regulated expression of b3 integrin induced by dengue virus serotype 2 infection associated with virus entry into human dermal microvascular endothelial cells
Zhang, J. l. / Wang, J. l. / Gao, N. / Chen, Z. t. / Tian, Y. p. / An, J. | 2007
769
A particular hydrophobic cluster in the residual structure of reduced lysozyme drastically affects the amyloid fibrils formation
Mishima, Tomonori / Ohkuri, Takatoshi / Monji, Akira / Imoto, Taiji / Ueda, Tadashi | 2007
773
Synergistic cooperation of Sall4 and Cyclin D1 in transcriptional repression
Böhm, Johann / Kaiser, Frank J. / Borozdin, Wiktor / Depping, Reinhard / Kohlhase, Jürgen | 2007
780
Insulin-like growth factor 1 enhances the migratory capacity of mesenchymal stem cells
Li, Yangxin / Yu, XiYong / Lin, ShuGuang / Li, XiaoHong / Zhang, Saidan / Song, Yao-Hua | 2007
785
A cis-element in the 3′-untranslated region of IκB-ζ mRNA governs its stimulus-specific expression
Watanabe, Satoshi / Takeshige, Koichiro / Muta, Tatsushi | 2007
785
A cis-element in the 3prime-untranslated region of IkB-z mRNA governs its stimulus-specific expression
Watanabe, S. / Takeshige, K. / Muta, T. | 2007
792
Expression and initial characterization of human ALDH3B1
Marchitti, Satori A. / Orlicky, David J. / Vasiliou, Vasilis | 2007
799
Unprocessed barley aleurone endo-β-1,4-xylanase X-I is an active enzyme
Van Campenhout, Steven / Pollet, Annick / Bourgois, Tine M. / Rombouts, Sigrid / Beaugrand, Johnny / Gebruers, Kurt / De Backer, Evelien / Courtin, Christophe M. / Delcour, Jan A. / Volckaert, Guido | 2007
799
Unprocessed barley aleurone endo-b-1,4-xylanase X-I is an active enzyme
Van Campenhout, S. / Pollet, A. / Bourgois, T. M. / Rombouts, S. / Beaugrand, J. / Gebruers, K. / De Backer, E. / Courtin, C. M. / Delcour, J. A. / Volckaert, G. | 2007
805
The expression of the mitochondrial gene MT-ND4 is downregulated in cystic fibrosis
Valdivieso, Ángel G. / Marcucci, Florencia / Taminelli, Guillermo / Guerrico, Anatilde González / Álvarez, Sergio / Teiber, María Luz / Dankert, Marcelo A. / Santa-Coloma, Tomás A. | 2007
810
Effect of thioredoxin reductase 1 on glucocorticoid receptor activity in human outer root sheath cells
Sohn, Kyung-Cheol / Jang, Sunhyae / Choi, Dae-Kyoung / Lee, Young-Sook / Yoon, Tae-Jin / Jeon, Eun Kyoung / Kim, Kyung Ho / Seo, Young-Joon / Lee, Jeung-Hoon / Park, Jang-Kyu et al. | 2007
816
Mutational analysis of the N-glycosylation sites of Duffy antigen/receptor for chemokines
Czerwinski, Marcin / Kern, Joanna / Grodecka, Magdalena / Paprocka, Maria / Krop-Watorek, Anna / Wasniowska, Kazimiera | 2007
822
Corrigendum to “Functional characterization of the human solute carrier, SLC41A2” [Biochem. Biophys. Res. Commun. 330 (2005) 701–705]
Goytain, Angela / Quamme, Gary A. | 2005
IFC
Editorial Board
| 2007

Similar titles