Silk fibroin nanoparticles and drug-loading silk fibroin nanoparticles (Chinese)

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The invention provides silk fibroin nanoparticles and drug-loading silk fibroin nanoparticles. The silk fibroin nanoparticles are prepared by a method comprising the following steps: dissolving silk fibroin in water, and keeping the water in which the silk fibroin is dissolved at 2-6 DEG C for 2-8h, so that a silk fibroin solution is obtained; mixing the silk fibroin solution with an organic solvent at the volume ratio of 1 to (4-8), and implementing stirring so as to obtain a nanoparticle suspension, wherein the organic solvent is selected from ethanol, isopropanol or acetone; implementing centrifugal separation on the nanoparticle suspension, washing the nanoparticle suspension by virtue of de-ionized water, and implementing centrifuging, so that a nanoparticle precipitate is obtained; and dispersing the nanoparticle precipitate by virtue of de-ionized water, so that the silk fibroin nanoparticles are obtained. The drug-loading silk fibroin nanoparticles can improve intake of drugs in cells and prolong retention time of the drugs; the drug-loading silk fibroin nanoparticles have the advantages of being effective, long-acting and targeted in cells and the drug-loading silk fibroin nanoparticles are conducive to the improvement of bioavailability of the drugs; the silk fibroin nanoparticles are excellent in biological adhesion and free from immunogenicity; and the silk fibroin nanoparticles are free from obvious inflammatory response and tissue fibrosis, and are excellent in biocompatibility and safety.

本发明提供了种丝素蛋白纳米粒和载药丝素蛋白纳米粒,丝素蛋白纳米粒由以下方法制得:将丝素蛋白溶于水中,在2~6℃下静置2~8h,得到丝素蛋白溶液;将体积比为1:4~8的丝素蛋白溶液和有机溶剂混合,搅拌得到纳米粒混悬液,有机溶剂选自乙醇、异丙醇或丙酮;将所述纳米粒混悬液离心分离,去离子水洗涤,离心得到纳米粒沉淀;去离子水分散该纳米粒沉淀得到丝素蛋白纳米粒。载药后的丝素蛋白纳米粒可提高药物在细胞内的摄取量,延长药物的滞留时间,具有胞内高效、长效及靶向的优势,有利于提高药物的生物利用度;具有良好的生物粘附性,且无免疫原性,不会引起明显的炎症反应和组织纤维化,生物相容性及安全性好。

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  • Title:
    Silk fibroin nanoparticles and drug-loading silk fibroin nanoparticles
  • Additional title:
    种丝素蛋白纳米粒和载药丝素蛋白纳米粒
  • Patent number:
    CN107157952
  • Application date:
    2017-06-30
  • Patent applicant:
  • Patent family:
  • Author / Creator:
  • Publisher:
    Europäisches Patentamt
  • Year of publication:
    2017
  • Type of media:
    Patent
  • Type of material:
    Electronic Resource
  • Language:
    Chinese
  • Classification:
    IPC:    A61K Zubereitungen für medizinische, zahnärztliche oder kosmetische Zwecke, PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES / A61P Spezifische therapeutische Aktivität von chemischen Verbindungen oder medizinischen Zubereitungen, SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

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