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    A rapid extraction method for glycogen from formalin-fixed liver (English)

    In: Carbohydrate polymers ; 118 ; 9-15 ; 2015
    • ISSN:
      0144-8617
    • Article (Journal)  /  Print

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    Liver glycogen, a highly branched polymer, acts as our blood-glucose buffer. While past structural studies have extracted glycogen from fresh or frozen tissue using a cold-water, sucrose-gradient centrifugation technique, a method for the extraction of glycogen from formalin-fixed liver would allow the analysis of glycogen from human tissues that are routinely collected in pathology laboratories. In this study, both sucrose-gradient and formalin-fixed extraction techniques were carried out on piglet livers, with the yields, purities and size distributions (using size exclusion chromatography) compared. The formalin extraction technique, when combined with a protease treatment, resulted in higher yields (but lower purities) of glycogen with size distributions similar to the sucrose-gradient centrifugation technique. This formalin extraction procedure was also significantly faster, allowing glycogen extraction throughput to increase by an order of magnitude. Both extraction techniques were compatible with mass spectrometry proteomics, with analysis showing the two techniques were highly complementary.

    Table of contents – Volume 118

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    The tables of contents are generated automatically and are based on the data records of the individual contributions available in the index of the TIB portal. The display of the Tables of Contents may therefore be incomplete.

    1
    A comparative study on properties of micro and nanopapers produced from cellulose and cellulose nanofibres
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    9
    A rapid extraction method for glycogen from formalin-fixed liver
    Sullivan, Mitchell A. / Li, Shihan / Aroney, Samuel T.N. / Deng, Bin / Li, Cheng / Roura, Eugeni / Schulz, Benjamin L. / Harcourt, Brooke E. / Forbes, Josephine M. / Gilbert, Robert G. et al. | 2014
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    24
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    30
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    36
    Characterization of hydroxypropyl-β-cyclodextrins with different substitution patterns via FTIR, GC–MS, and TG–DTA
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    41
    Preparation of the oligosaccharides derived from Flammulina velutipes and their antioxidant activities
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    44
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    52
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    83
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    115
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    119
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    133
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    143
    Properties of alginate fiber spun-dyed with fluorescent pigment dispersion
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    150
    Crystalline characteristics of cellulose fiber and film regenerated from ionic liquid solution
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    156
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    165
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    170
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    183
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    199
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    209
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    218
    Synthesis, anti-oxidant activity, and biodegradability of a novel recombinant polysaccharide derived from chitosan and lactose
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    224
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    235
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    257
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    IFC
    Inside front cover
    | 2014

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