On the role of isoleucyl-tRNA synthetase in multivalent repression (English)

National licence
in Biochemical Genetics ; 6 , 2 ; 99-118
Biochemical Genetics

Abstract

An isoleucine auxotroph of Salmonella typhimurium was derived from a merodiploid strain (containing the F′-14 episome from Escherichia coli) that contained two copies of the structural genes concerned with isoleucine and valine biosynthesis. A haploid derivative, strain TU6001, having the same growth properties as the original merodiploid mutant was found to have normal biosynthetic enzymes and an altered isoleucyl-tRNA synthetase. The K m for isoleucine was increased by about 200-fold over that for the wild-type enzyme. All five enzymes in the isoleucine and valine biosynthetic pathway were derepressed relative to wild-type enzyme levels. A partial revertant of strain TU6001 was isolated which had properties that were intermediate between those of the mutant and the wild type (i.e., intermediate growth dependence on exogenous isoleucine, intermediate activity of isoleucyl-tRNA synthetase, and intermediate derepression of biosynthetic enzymes). The properties of strain TU6001 were demonstrated to be simultaneously transferable by transduction (using PLT22 H4 bacteriophage) of a single genetic locus, linked to pyr A, which has been designated ilv S. It is concluded that some function of the isoleucyl-tRNA synthetase is important in repression of the isoleucine and valine biosynthetic enzymes.

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Table of contents – Volume 6, Issue 2

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Tables of content are generated automatically and are based on records of articles contained that are available in the TIB-Portal index. Due to missing records of articles, the volume display may be incomplete, even though the whole journal is available at TIB.

99
On the role of isoleucyl-tRNA synthetase in multivalent repression
Blatt, Joel M. / Umbarger, H. E. | 1972
119
Glucose 6-phosphate dehydrogenase: Identity of erythrocyte and leukocyte enzyme with report of a new variant in Chinese
Chan, T. K. / Todd, D. / Lai, M. C. S. | 1972
125
Esterase polymorphisms in Microtus ochrogaster: interaction and linkage
Semeonoff, Robert | 1972
139
Electrophoretic properties of hypoxanthine-guanine phosphoribosyl transferase in erythrocytes of subjects with Lesch-Nyhan syndrome
Bakay, Bohdan / Nyhan, William L. | 1972
147
Genetic variation of phosphoglucose isomerase in some hystricomorph rodents
Carter, Nicholas D. / Hill, Michael R. / Weir, Barbara J. | 1972
157
The genetics of aryl hydrocarbon hydroxylase induction in mice: A single gene difference between C57BL/6J and DBA/2J
Thomas, Paul E. / Kouri, Richard E. / Hutton, John J. | 1972
169
Activity variation between and within two soybean trypsin inhibitor electrophoretic forms
Clark, R. W. / Hymowitz, T. | 1972
183
On the homologous relationship between the variable and the constant parts of immunoglobulins
Urbain, J. | 1972
195
The mouse liver aldehyde oxidase locus (Aox)
Watson, J. G. / Higgins, T. J. / Collins, P. B. / Chaykin, S. | 1972
205
Induction of null-activity mutants for the acid phosphatase-1 gene in Drosophila melanogaster
Bell, John B. / MacIntyre, Ross J. / Olivieri, Allan P. | 1972
217
Levels of RNA and DNA in Drosophila melanogaster at different stages of development: A comparison between one bobbed and two phenotypically non-bobbed stocks
Howells, A. J. | 1972
231
Molecular pathology of ceruloplasmin
Neifakh, S. A. / Vasiletz, I. M. / Shavlovsky, M. M. | 1972

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