Detection of Aflatoxin B1 with a Label-Free Ellipsometry Immunosensor (English)

In: BioNanoScience   ;  1 ,  1  ;  38-45  ;  2011

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Abstract The method of spectroscopic ellipsometry in total internal reflection mode (TIRE) was utilised for detection of Aflatoxin B1. The method of TIRE with the improved data analysis was capable of detection of aflatoxin molecules in low concentrations (down to 0.04 ng/ml) using a label-free and cost-effective direct immunoassay format. TIRE study of the binding kinetics yielded a large value of the association constant in the range of 106 l mol−1 which is typical for highly specific immune reactions. The comparison of the experimental data for three mycotoxins studied (e.g. aflatoxin B1, T-2 mycotoxin, and zearalenone) confirmed a common mechanism of the sensitivity boost due to the aggregation of hydrophobic molecules of mycotoxins in aqueous solutions.

Table of contents – Volume 1, Issue 1

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1
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Material Memristive Device Circuits with Synaptic Plasticity: Learning and Memory
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Self-Assembled Peptide Nanotubes as an Etching Material for the Rapid Fabrication of Silicon Wires
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38
Detection of Aflatoxin B1 with a Label-Free Ellipsometry Immunosensor
Nabok, Alexei V. / Mustafa, Mohd Kamarulzaki / Tsargorodskaya, Anna / Starodub, Nikolaj F. | 2011
46
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53
Structure, Scaling, and Performance of Natural Micro- and Nanocomposites
Bekah, Sacheen / Rabiei, Reza / Barthelat, Francois | 2011
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