Electroanalysis of Cytochrome P450 3A4 Catalytic Properties with Nanostructured Electrodes: The Influence of Vitamin B Group on Diclofenac Metabolism (English)

Vitamin B Group Modulates Pharmacological Action of Diclofenac and Exerts Influence on the Electrocatalytic Activity of Cytochrome P450 3A4

In: BioNanoScience   ;  1 ,  1  ;  46-52  ;  2011

How to get this document?

Download
Commercial Copyright fee: €34.95 Basic fee: €4.00 Total price: €38.95
Academic Copyright fee: €12.50 Basic fee: €2.00 Total price: €14.50

Abstract Application of vitamin B group permits to shorten the longitude of diclofenac (DF) therapy and to reduce the daytime dose of this drug. All the three schemes of treatment with diclofenac—i.e., diclofenac alone, diclofenac plus two tablets of Gitagamp (composition of vitamin B group), and diclofenac plus four tablets of Gitagamp—enabled to achieve maximum diclofenac concentration in blood within 1 h after its uptake. In the case of diclofenac administration, C max corresponds to 1,137.2 ± 82.4 ng/ml (3.41 ± 0.25 μM); with two tablets of Gitagamp, it corresponds to 1,326.7 ± 122.5 ng/ml (3.97 ± 0.37 μM), and with four tablets, it corresponds to 2,200.4 ± 111.3 ng/ml (6.59 ± 0.33 μM). Pharmacodynamics and pharmacokinetics data were confirmed in electrochemical experiments with cytochrome P450 3A4 and electrodes nanostructured with gold nanoparticles, stabilized by the synthetic membrane-like surfactant didodecyldimethylammonium bromide. Electrochemical analysis revealed the influence of vitamin B group on the metabolism of the nonsteroidal anti-inflammatory drug diclofenac catalyzed by cytochrome P450 3A4. Riboflavin was found to be the most effective inhibitor of diclofenac hydroxylation by cytochrome P450 3A4, as was demonstrated by comparing the influence of vitamin B group (B1, B2, and B6) at the same (300 μM) concentration. The data obtained provide evidence for the possible regulation of pharmacokinetic parameters and pharmacodynamic effects for DF through the influence of vitamin B group on the catalytic activity of cytochrome P450 3A4.

Table of contents – Volume 1, Issue 1

Show all volumes and issues

The tables of contents are generated automatically and are based on the data records of the individual contributions available in the index of the TIB portal. The display of the Tables of Contents may therefore be incomplete.

1
New Insight on Bio-sensing by Nano-fabricated Memristors
Sacchetto, Davide / Doucey, Marie-Agnès / Micheli, Giovanni / Leblebici, Yusuf / Carrara, Sandro | 2011
4
Influence of Gold Nanoparticles on Radiation Dose Enhancement and Cellular Migration in Microbeam-Irradiated Cells
Rahman, Wan Nordiana / Davidson, Robert / Yagi, Naoto / Bansal, Vipul / Geso, Moshi / Darby, Ian | 2011
14
Dynamic Failure of a Lamina Meshwork in Cell Nuclei under Extreme Mechanical Deformation
Qin, Zhao / Buehler, Markus J. | 2011
24
Material Memristive Device Circuits with Synaptic Plasticity: Learning and Memory
Erokhin, Victor / Berzina, Tatiana / Camorani, Paolo / Smerieri, Anteo / Vavoulis, Dimitris / Feng, Jianfeng / Fontana, Marco P. | 2011
31
Self-Assembled Peptide Nanotubes as an Etching Material for the Rapid Fabrication of Silicon Wires
Larsen, Martin B. / Andersen, Karsten B. / Svendsen, Winnie E. / Castillo-León, Jaime | 2011
38
Detection of Aflatoxin B1 with a Label-Free Ellipsometry Immunosensor
Nabok, Alexei V. / Mustafa, Mohd Kamarulzaki / Tsargorodskaya, Anna / Starodub, Nikolaj F. | 2011
46
Electroanalysis of Cytochrome P450 3A4 Catalytic Properties with Nanostructured Electrodes: The Influence of Vitamin B Group on Diclofenac Metabolism
Makhova, Anna A. / Shumyantseva, Victoria V. / Shich, Evgeniya V. / Bulko, Tatyana V. / Kukes, Vladimir G. / Sizova, Oksana S. / Ramenskaya, Galina V. / Usanov, Sergei A. / Archakov, Alexander I. | 2011
53
Structure, Scaling, and Performance of Natural Micro- and Nanocomposites
Bekah, Sacheen / Rabiei, Reza / Barthelat, Francois | 2011
Feedback