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Based on a long-span pre-stressed concrete continuous rigid frame bridge, the Chaoyangsi bridge, the influence on stress and deformation of the girder caused by different closure temperature was analyzed. With the aided platform Midas/Civil, comparative analysis of three different closure temperatures was carried on to determine the reasonable one. It is concluded that different closure temperature has important influence on girder stress, but the most dangerous stress combination of girder under serviceability limit state could be taken as remain the same. Though different closure temperature has little influence on the displacement of girder, it is recommended to pay highly attention to the influence on the mid-span vertical displacement when doing the formwork. And finally this article puts forward some effective measures to prevent and reduce the temperature effect.