Effects of rapamycin on number activity and eNOS of endothelial progenitor cells from peripheral blood (English)

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in Cell Proliferation ; 39 , 2 ; 117-125
Cell Proliferation

Abstract.  The aim of this investigation is to determine whether rapamycin treatment has any effect on endothelial progenitor cells (EPCs). Total mononuclear cells (MNCs) were isolated from peripheral blood by Ficoll density gradient centrifugation, and then the cells were plated on fibronectin‐coated culture dishes. After 7 days in culture, attached cells were stimulated with rapamycin (in a series of final concentrations: 0.1, 1.0, 2.0 and 5.0 g/ml) for 6, 12, 24 and 48 h. EPCs were characterized as adherent cells, double positive for DiLDL uptake and lectin binding by direct fluorescence staining. EPC proliferation and migration were determined using the MTT assay and a modified version of the Boyden chamber assay, respectively. An EPC adhesion assay was performed by replating the cells on fibronectin‐coated dishes; adherent cells were then counted. Tube formation activity was assayed by using a tube formation assay kit and endothelial nitric oxide synthase (eNOS) was assayed by Western blot analysis. Incubation of isolated human MNCs with rapamycin decreased the number of EPCs present; rapamycin also decreased EPCs proliferative, migratory, adhesive, tube formation capacity and eNOS production in a concentration‐ and time‐dependent manner. Rapamycin was found to decrease the number, proliferative, migratory, adhesive and tube formation capacities of the EPCs, and also was found to decreases eNOS in the EPCs.

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Table of contents – Volume 39, Issue 2

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Tables of content are generated automatically and are based on records of articles contained that are available in the TIB-Portal index. Due to missing records of articles, the volume display may be incomplete, even though the whole journal is available at TIB.

75
Predictive medicine and clinical cytomics research: résumé of the 15th Annual Meeting of the German Society for Cytometry (Deutsche Gesellschaft für Zytometrie, DGfZ)
Brockhoff, G. / Müller, S. / Sarraf, C. / Tarnok, A. | 2006
75
Predictive medicine and clinical cytomics research: resume of the 15th Annual Meeting of the German Society for Cytometry (Deutsche Gesellschaft fur Zytometrie, DGfZ)
Brockhoff, G. / Muller, S. / Sarraf, C. / Tarnok, A. | 2006
79
Proliferation and pluripotency potential of ectomesenchymal cells derived from first branchial arch
Lin, Yunfeng / Yan, Zhengbin / Liu, Lei / Qiao, Ju / Jing, Wei / Wu, Ling / Chen, Xizhe / Li, Zhiyong / Tang, Wei / Zheng, Xiaohui et al. | 2006
93
A novel type II membrane receptor up-regulated by IFN-a in fibroblasts functions in cell proliferation through the JAK-STAT signalling pathway
Liu, L. D. / Dong, C. H. / Shi, H. J. / Zhao, H. L. / Wang, L. C. / Ma, S. H. / Li, Q. H. | 2006
93
A novel type II membrane receptor up‐regulated by IFN‐α in fibroblasts functions in cell proliferation through the JAK‐STAT signalling pathway
Liu, L.‐D. / Dong, C.‐H. / Shi, H.‐J. / Zhao, H.‐L. / Wang, L.‐C. / Ma, S.‐H. / Li, Q.‐H. | 2006
105
Early induction of TGF-b1 through a fasting-re-feeding regimen promotes liver carcinogenesis by a sub-initiating dose of diethylnitrosamine
Tessitore, L. / Bollito, E. | 2006
105
Early induction of TGF‐β1 through a fasting–re‐feeding regimen promotes liver carcinogenesis by a sub‐initiating dose of diethylnitrosamine
Tessitore, L. / Bollito, E. | 2006
117
Effects of rapamycin on number activity and eNOS of endothelial progenitor cells from peripheral blood
Chen, T.‐G. / Chen, J. Z. / Wang, X.‐X. | 2006
127
Not only insulin stimulates mitochondriogenesis in muscle cells, but mitochondria are also essential for insulin‐mediated myogenesis
Pawlikowska, Patrycja / Gajkowska, Barbara / Hocquette, Jean‐François / Orzechowski, Arkadiusz | 2006
147
The historical analysis of aspirin discovery, its relation to the willow tree and antiproliferative and anticancer potential
Mahdi, J. G. / Mahdi, A. J. / Bowen, I. D. | 2006

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