MOUSE STRAIN‐SPECIFIC DIFFERENCES IN CARDIAC METABOLIC ENZYME ACTIVITIES OBSERVED IN A MODEL OF ISOPROTERENOL‐INDUCED CARDIAC HYPERTROPHY (English)

Clinical and Experimental Pharmacology and Physiology

Alterations in myocardial energy metabolism accompany pressure overload‐induced hypertrophy. We previously described a novel model of catecholamine‐induced hypertrophy in which A/J mice exhibit more robust cardiac hypertrophy than B6 mice. Accordingly, we assessed the influence of mouse strain on the activities of key myocardial metabolic enzymes and whether there are strain‐related metabolic adaptations to short‐term, high‐dose isoproterenol (ISO) administration.

Thirty‐nine male mice (19 A/J mice, 20 B6 mice), aged 12–15 weeks, were randomly assigned to receive either ISO (100 mg/kg, s.c.) or vehicle (sterile water) daily for 5 days. On Day 6, all hearts were excised, weighed, freeze clamped and assayed for pyruvate dehydrogenase (PDH), medium chain acyl‐CoA dehydrogenase, carnitine palmitoyl transferase I and citrate synthase activities. Plasma fatty acids (FA) were also measured.

The ISO‐treated A/J mice demonstrated greater percentage increases in gravimetric heart weight/bodyweight ratio than ISO‐treated B6 mice (24 vs 3%, respectively; P <� 0.001). All enzyme activities were significantly greater in vehicle‐treated B6 mice than in A/J mice, illustrating a greater capacity for aerobic metabolism in B6 mice. Administration of ISO reduced PDHa (active form) activity in B6 mice by 47% (P < 0.001), with no significant change seen in A/J mice. Free FA levels were not significantly different between groups; thus, the differences in PDHa were not due to changes in FA.

The basal activity of myocardial metabolic enzymes is greater in B6 mice than in A/J mice and ISO alters myocardial PDH activity in a mouse strain‐dependent manner. Compared with A/J mice, B6 mice demonstrate less ISO‐induced cardiac hypertrophy, but greater activity of key enzymes regulating FA and carbohydrate oxidation, which may protect against the development of hypertrophy. The metabolic adaptations associated with ISO‐induced hypertrophy differ from those reported with pressure overload hypertrophy.

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Table of contents – Volume 34, Issue 1-2

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Tables of content are generated automatically and are based on records of articles contained that are available in the TIB-Portal index. Due to missing records of articles, the volume display may be incomplete, even though the whole journal is available at TIB.

1
SLEEP AND METABOLIC CONTROL: WAKING TO A PROBLEM?
Trenell, Michael I / Marshall, Nathaniel S / Rogers, Naomi L | 2007
10
EFFECT OF BAY 41‐2272 IN THE PULMONARY HYPERTENSION INDUCED BY HEPARIN–PROTAMINE COMPLEX IN ANAESTHETIZED DOGS
Freitas, Cristiane F / Morganti, Rafael P / Annichino‐Bizzacchi, Joyce M / De Nucci, Gilberto / Antunes, Edson | 2007
15
Ca2+‐DEPENDENT DIFFERENTIAL DEVELOPMENT OF CARBACHOL‐INDUCED DESENSITIZATION TO RECEPTOR AGONISTS AND HIGH K+ IN GUINEA‐PIG TAENIA CAECI
Hishinuma, Shigeru / Matsumoto, Yukio / Sato, Ryo / Saito, Masaki | 2007
22
THE SPECIFIC FREE RADICAL SCAVENGER EDARAVONE SUPPRESSES BLEOMYCIN‐INDUCED ACUTE PULMONARY INJURY IN RABBITS
Asai, Toshihiro / Ohno, Yasushi / Minatoguchi, Shinya / Funaguchi, Norihiko / Yuhgetsu, Hideyuki / Sawada, Masahiro / Takemura, Genzou / Komada, A / Fujiwara, Takako / Fujiwara, Hisayoshi | 2007
27
EFFECTS OF FENOFIBRATE AND XUEZHIKANG ON HIGH‐FAT DIET‐INDUCED NON‐ALCOHOLIC FATTY LIVER DISEASE
Hong, Xue Zhi / Li, Lian Da / Wu, Li Mao | 2007
36
CYCLO‐OXYGENASE‐2 INHIBITION ATTENUATES THE PROGRESSION OF NEPHROPATHY IN UNINEPHRECTOMIZED DIABETIC RATS
Komers, Radko / Lindsley, Jessie N / Oyama, Terry T / Anderson, Sharon | 2007
42
EFFECT OF α1‐ADRENOCEPTOR SUBTYPE‐SELECTIVE INVERSE AGONISTS ON NON‐PREGNANT AND LATE‐PREGNANT CERVICAL RESISTANCE IN VITRO IN THE RAT
Kolarovszki‐Sipiczki, Zoltán / Gáspár, Róbert / Ducza, Eszter / Páldy, Eszter / Benyhe, Sándor / Borsodi, Anna / Falkay, George | 2007
42
EFFECT OF a1-ADRENOCEPTOR SUBTYPE-SELECTIVE INVERSE AGONISTS ON NON-PREGNANT AND LATE-PREGNANT CERVICAL RESISTANCE IN VITRO IN THE RAT:
Kolarovszki-Sipiczki, Z. / Gaspar, R. / Ducza, E. / Paldy, E. / Benyhe, S. / Borsodi, A. / Falkay, G. | 2007
48
IMPAIRED EFFECT OF SALT LOADING ON NITRIC OXIDE‐MEDIATED RELAXATION IN AORTAS FROM STROKE‐PRONE SPONTANEOUSLY HYPERTENSIVE RATS
Kagota, Satomi / Kubota, Yoko / Nejime, Namie / Nakamura, Kazuki / Kunitomo, Masaru / Shinozuka, Kazumasa | 2007
55
EFFECTS OF LANDIOLOL ON MECHANICAL AND METABOLIC CHANGES IN RAT REPERFUSED ISCHAEMIC HEARTS
Sakanashi, Makiko / Sakanashi, Mayuko / Sugahara, Kazuhiro / Sakanashi, Matao | 2007
61
ENDOTOXIN‐INDUCED ACUTE LUNG INJURY IS ENHANCED IN RATS WITH SPONTANEOUS HYPERTENSION
Liu, Demeral D / Hsiang Hsu, Yung / Chen, Hsing I | 2007
70
EFFECTS OF HYPERBARIC OXYGEN ON GLUCOSE, LACTATE, GLYCEROL AND ANTI‐OXIDANT ENZYMES IN THE SKELETAL MUSCLE OF RATS DURING ISCHAEMIA AND REPERFUSION
Bosco, Gerardo / Yang, Zhong‐jin / Nandi, Jyotirmoy / Wang, Jingping / Chen, Chung / Camporesi, Enrico M | 2007
77
MOUSE STRAIN‐SPECIFIC DIFFERENCES IN CARDIAC METABOLIC ENZYME ACTIVITIES OBSERVED IN A MODEL OF ISOPROTERENOL‐INDUCED CARDIAC HYPERTROPHY
Faulx, Michael D / Chandler, Margaret P / Zawaneh, Michael S / Stanley, William C / Hoit, Brian D | 2007
81
CARDIAC‐SPECIFIC OVEREXPRESSION OF CATALASE PROLONGS LIFESPAN AND ATTENUATES AGEING‐INDUCED CARDIOMYOCYTE CONTRACTILE DYSFUNCTION AND PROTEIN DAMAGE
Wu, Shan / Li, Qun / Du, Min / Li, Shi‐Yan / Ren, Jun | 2007
88
PULMONARY VASCULAR REACTIVITY OF SPONTANEOUSLY HYPERTENSIVE RATS IS EXACERBATED IN RESPONSE TO THE CENTRAL ADMINISTRATION OF EXOGENOUS NITRIC OXIDE
Schwenke, Daryl O / Pearson, James T / Tsuchimochi, Hirotsugu / Kangawa, Kenji / Shirai, Mikiyasu | 2007
95
ENDOGENOUS NITRIC OXIDE ATTENUATES β‐ADRENOCEPTOR‐MEDIATED RELAXATION IN RAT AORTA
Kang, Khong Bee / Van Der Zypp, Andrea / Majewski, Henryk | 2007
95
ENDOGENOUS NITRIC OXIDE ATTENUATES b-ADRENOCEPTOR-MEDIATED RELAXATION IN RAT AORTA:
Kang, K. B. / van der Zypp, A. / Majewski, H. | 2007
102
GENETIC POLYMORPHISMS OF CYTOCHROME P450 ENZYMES 2C9 AND 2C19 IN A HEALTHY IRANIAN POPULATION
Zand, Negin / Tajik, Nader / Moghaddam, Alireza Salek / Milanian, Iraj | 2007
106
INTERACTIONS BETWEEN METOCLOPRAMIDE AND MORPHINE: ENHANCED ANTINOCICEPTION AND MOTOR DYSFUNCTION IN RATS
Kamerman, Peter R / Becker, Nicole / Fick, Linda G | 2007
113
CARNITINE PALMITOYL TRANSFERASE‐I INHIBITION IS NOT ASSOCIATED WITH CARDIAC HYPERTROPHY IN RATS FED A HIGH‐FAT DIET
Okere, Isidore C / Chandler, Margaret P / McElfresh, Tracy A / Rennison, Julie H / Kung, Theodore A / Hoit, Brian D / Ernsberger, Paul / Young, Martin E / Stanley, William C | 2007
120
MECHANISMS MEDIATING SEROTONIN‐INDUCED CONTRACTION OF COLONIC MYOCYTES
Xu, Long / Yu, Bao‐Ping / Chen, Jian‐Guo / Luo, He‐Sheng | 2007
129
COMPARISON OF THE ELECTROPHYSIOLOGICAL PROPERTIES OF THE SHEEP ISOLATED COSTAL AND DIAPHRAGMATIC PARIETAL PLEURA
Zarogiannis, Sotirios / Hatzoglou, Chrissi / Stefanidis, Ioannis / Ioannou, Maria / Paraskeva, Efrosini / Gourgoulianis, Konstantinos / Molyvdas, Paschalis‐Adam | 2007
132
A MOUSE MODEL FOR ASSESSING THE IMPACT OF INGESTED NUTRIENTS ON GASTRIC EMPTYING RATE
Symonds, Erin L / Butler, Ross / Omari, Taher I | 2007

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