CARDIAC‐SPECIFIC OVEREXPRESSION OF CATALASE PROLONGS LIFESPAN AND ATTENUATES AGEING‐INDUCED CARDIOMYOCYTE CONTRACTILE DYSFUNCTION AND PROTEIN DAMAGE (English)

Clinical and Experimental Pharmacology and Physiology

Oxidative stress plays a role in senescence‐associated organ deterioration. This is supported by the beneficial effects of anti‐oxidants against ageing‐related organ damage, although their role in cardiac ageing has not been elucidated.

The aim of the present study was to examine the impact of cardiac‐specific overexpression of catalase, an enzyme for H2O2 detoxification, on cardiac contractile function and protein damage in young (3–4 months) and old (26–28 months) male mice. Lifespan was analysed using the Kaplan–Meier survival curve. Cardiomyocyte contractile indices at various stimulus frequencies (0.1–5.0 Hz) were analysed, including peak shortening (PS), time to 90% PS, time to 90% relengthening (TR90) and maximal velocity of shortening/relengthening (±dL/dt). Protein damage was assessed using protein carbonyl formation. Catalase transgenic mice showed longer lifespan than wild‐type FVB mice. The catalase transgene itself did not alter bodyweight or organ weight, or myocyte function. Ageing depressed ±dL/dt and prolonged TR90, but had no effect on other indices in FVB mice. Increased frequency triggered decreases in PS amplitude were exaggerated in aged FVB myocytes. Interestingly, ageing‐induced mechanical defects were significantly attenuated in myocytes from catalase mice. Protein carbonyl formation was elevated in aged FVB compared with young FVB mice, which was significantly diminished in catalase mice. The proteomes of the myocardium of young or old FVB and catalase mice were compared using two‐dimensional gel electrophoresis and mass spectrometry. Six proteins with differential expression between yound and old FVB groups were tentatively identified, some of which were reversed by catalase.

In summary, the present data suggest that catalase protects cardiomyocytes from ageing‐induced contractile defects and protein damage.

How to get this document?
Download
Commercial Copyright fee: €37.76 Basic fee: €4.00 Total price: €41.76
Academic Copyright fee: €37.76 Basic fee: €2.00 Total price: €39.76

Document information


Table of contents – Volume 34, Issue 1-2

Show all volumes and issues

Tables of content are generated automatically and are based on records of articles contained that are available in the TIB-Portal index. Due to missing records of articles, the volume display may be incomplete, even though the whole journal is available at TIB.

1
SLEEP AND METABOLIC CONTROL: WAKING TO A PROBLEM?
Trenell, Michael I / Marshall, Nathaniel S / Rogers, Naomi L | 2007
10
EFFECT OF BAY 41‐2272 IN THE PULMONARY HYPERTENSION INDUCED BY HEPARIN–PROTAMINE COMPLEX IN ANAESTHETIZED DOGS
Freitas, Cristiane F / Morganti, Rafael P / Annichino‐Bizzacchi, Joyce M / De Nucci, Gilberto / Antunes, Edson | 2007
15
Ca2+‐DEPENDENT DIFFERENTIAL DEVELOPMENT OF CARBACHOL‐INDUCED DESENSITIZATION TO RECEPTOR AGONISTS AND HIGH K+ IN GUINEA‐PIG TAENIA CAECI
Hishinuma, Shigeru / Matsumoto, Yukio / Sato, Ryo / Saito, Masaki | 2007
22
THE SPECIFIC FREE RADICAL SCAVENGER EDARAVONE SUPPRESSES BLEOMYCIN‐INDUCED ACUTE PULMONARY INJURY IN RABBITS
Asai, Toshihiro / Ohno, Yasushi / Minatoguchi, Shinya / Funaguchi, Norihiko / Yuhgetsu, Hideyuki / Sawada, Masahiro / Takemura, Genzou / Komada, A / Fujiwara, Takako / Fujiwara, Hisayoshi | 2007
27
EFFECTS OF FENOFIBRATE AND XUEZHIKANG ON HIGH‐FAT DIET‐INDUCED NON‐ALCOHOLIC FATTY LIVER DISEASE
Hong, Xue Zhi / Li, Lian Da / Wu, Li Mao | 2007
36
CYCLO‐OXYGENASE‐2 INHIBITION ATTENUATES THE PROGRESSION OF NEPHROPATHY IN UNINEPHRECTOMIZED DIABETIC RATS
Komers, Radko / Lindsley, Jessie N / Oyama, Terry T / Anderson, Sharon | 2007
42
EFFECT OF a1-ADRENOCEPTOR SUBTYPE-SELECTIVE INVERSE AGONISTS ON NON-PREGNANT AND LATE-PREGNANT CERVICAL RESISTANCE IN VITRO IN THE RAT:
Kolarovszki-Sipiczki, Z. / Gaspar, R. / Ducza, E. / Paldy, E. / Benyhe, S. / Borsodi, A. / Falkay, G. | 2007
42
EFFECT OF α1‐ADRENOCEPTOR SUBTYPE‐SELECTIVE INVERSE AGONISTS ON NON‐PREGNANT AND LATE‐PREGNANT CERVICAL RESISTANCE IN VITRO IN THE RAT
Kolarovszki‐Sipiczki, Zoltán / Gáspár, Róbert / Ducza, Eszter / Páldy, Eszter / Benyhe, Sándor / Borsodi, Anna / Falkay, George | 2007
48
IMPAIRED EFFECT OF SALT LOADING ON NITRIC OXIDE‐MEDIATED RELAXATION IN AORTAS FROM STROKE‐PRONE SPONTANEOUSLY HYPERTENSIVE RATS
Kagota, Satomi / Kubota, Yoko / Nejime, Namie / Nakamura, Kazuki / Kunitomo, Masaru / Shinozuka, Kazumasa | 2007
55
EFFECTS OF LANDIOLOL ON MECHANICAL AND METABOLIC CHANGES IN RAT REPERFUSED ISCHAEMIC HEARTS
Sakanashi, Makiko / Sakanashi, Mayuko / Sugahara, Kazuhiro / Sakanashi, Matao | 2007
61
ENDOTOXIN‐INDUCED ACUTE LUNG INJURY IS ENHANCED IN RATS WITH SPONTANEOUS HYPERTENSION
Liu, Demeral D / Hsiang Hsu, Yung / Chen, Hsing I | 2007
70
EFFECTS OF HYPERBARIC OXYGEN ON GLUCOSE, LACTATE, GLYCEROL AND ANTI‐OXIDANT ENZYMES IN THE SKELETAL MUSCLE OF RATS DURING ISCHAEMIA AND REPERFUSION
Bosco, Gerardo / Yang, Zhong‐jin / Nandi, Jyotirmoy / Wang, Jingping / Chen, Chung / Camporesi, Enrico M | 2007
77
MOUSE STRAIN‐SPECIFIC DIFFERENCES IN CARDIAC METABOLIC ENZYME ACTIVITIES OBSERVED IN A MODEL OF ISOPROTERENOL‐INDUCED CARDIAC HYPERTROPHY
Faulx, Michael D / Chandler, Margaret P / Zawaneh, Michael S / Stanley, William C / Hoit, Brian D | 2007
81
CARDIAC‐SPECIFIC OVEREXPRESSION OF CATALASE PROLONGS LIFESPAN AND ATTENUATES AGEING‐INDUCED CARDIOMYOCYTE CONTRACTILE DYSFUNCTION AND PROTEIN DAMAGE
Wu, Shan / Li, Qun / Du, Min / Li, Shi‐Yan / Ren, Jun | 2007
88
PULMONARY VASCULAR REACTIVITY OF SPONTANEOUSLY HYPERTENSIVE RATS IS EXACERBATED IN RESPONSE TO THE CENTRAL ADMINISTRATION OF EXOGENOUS NITRIC OXIDE
Schwenke, Daryl O / Pearson, James T / Tsuchimochi, Hirotsugu / Kangawa, Kenji / Shirai, Mikiyasu | 2007
95
ENDOGENOUS NITRIC OXIDE ATTENUATES b-ADRENOCEPTOR-MEDIATED RELAXATION IN RAT AORTA:
Kang, K. B. / van der Zypp, A. / Majewski, H. | 2007
95
ENDOGENOUS NITRIC OXIDE ATTENUATES β‐ADRENOCEPTOR‐MEDIATED RELAXATION IN RAT AORTA
Kang, Khong Bee / Van Der Zypp, Andrea / Majewski, Henryk | 2007
102
GENETIC POLYMORPHISMS OF CYTOCHROME P450 ENZYMES 2C9 AND 2C19 IN A HEALTHY IRANIAN POPULATION
Zand, Negin / Tajik, Nader / Moghaddam, Alireza Salek / Milanian, Iraj | 2007
106
INTERACTIONS BETWEEN METOCLOPRAMIDE AND MORPHINE: ENHANCED ANTINOCICEPTION AND MOTOR DYSFUNCTION IN RATS
Kamerman, Peter R / Becker, Nicole / Fick, Linda G | 2007
113
CARNITINE PALMITOYL TRANSFERASE‐I INHIBITION IS NOT ASSOCIATED WITH CARDIAC HYPERTROPHY IN RATS FED A HIGH‐FAT DIET
Okere, Isidore C / Chandler, Margaret P / McElfresh, Tracy A / Rennison, Julie H / Kung, Theodore A / Hoit, Brian D / Ernsberger, Paul / Young, Martin E / Stanley, William C | 2007
120
MECHANISMS MEDIATING SEROTONIN‐INDUCED CONTRACTION OF COLONIC MYOCYTES
Xu, Long / Yu, Bao‐Ping / Chen, Jian‐Guo / Luo, He‐Sheng | 2007
129
COMPARISON OF THE ELECTROPHYSIOLOGICAL PROPERTIES OF THE SHEEP ISOLATED COSTAL AND DIAPHRAGMATIC PARIETAL PLEURA
Zarogiannis, Sotirios / Hatzoglou, Chrissi / Stefanidis, Ioannis / Ioannou, Maria / Paraskeva, Efrosini / Gourgoulianis, Konstantinos / Molyvdas, Paschalis‐Adam | 2007
132
A MOUSE MODEL FOR ASSESSING THE IMPACT OF INGESTED NUTRIENTS ON GASTRIC EMPTYING RATE
Symonds, Erin L / Butler, Ross / Omari, Taher I | 2007