INHIBITION OF NAD(P)H OXIDASE REDUCES FIBRONECTIN EXPRESSION IN STROKE‐PRONE RENOVASCULAR HYPERTENSIVE RAT BRAIN (English)

Clinical and Experimental Pharmacology and Physiology

The aim of the present study was to test the hypothesis that in vivo chronic inhibition of NAD(P)H oxidase reduces cerebrovascular fibronectin expression in stroke‐prone renovascular hypertensive rats (RHRSP).

The RHRSP model was induced by two clips and NAD(P)H oxidase was inhibited with apocynin. The mRNA and protein expression of NAD(P)H oxidase subunit p22phox in brains of RHRSP and Sprague‐Dawley (control) rats was determined using real‐time reverse transcription–polymerase chain reaction, western blot and immunohistochemistry. The expression of fibronectin protein was localized immunohistochemically in cerebral vessels and then quantified by western blot.

Cerebrovascular fibronectin levels in RHRSP (n = 6) were significantly higher than control (n = 5) levels 8 weeks after operation (1.29 ± 0.04 vs 1.15 ± 0.02, respectively; P = 0.007). The p22phox immunopositive reactivity was localized in the cerebral vasculature of control rats and RHRSP. Furthermore, chronic treatment of RHRSP with apocynin, a selective NAD(P)H oxidase inhibitor, in the drinking water for 4 weeks (1.5 mmol/L, 5 weeks after operation) resulted in a significant decrease in the expression of p22phox protein (0.85 ± 0.01 vs 0.93 ± 0.01 in non‐treated RHRSP; n = 5; P = 0.002), with a concomitant reduction of fibronectin levels in the cerebral vasculature (1.31 ± 0.03 vs 1.56 ± 0.05 in non‐treated RHRSP; n = 5; P = 0.002). No significant differences were detected in the expression of p22phox mRNA and protein between RHRSP (4 and 8 weeks after renal artery constriction) and the control group.

These findings suggest that the chronic inhibition of NAD(P)H oxidase in vivo by apocynin reduces cerebrovascular fibronectin levels, which may lessen hypertensive cerebrovascular fibrosis.

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Table of contents – Volume 34, Issue 4

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Tables of content are generated automatically and are based on records of articles contained that are available in the TIB-Portal index. Due to missing records of articles, the volume display may be incomplete, even though the whole journal is available at TIB.

255
DIFFERENCES BETWEEN PATHOLOGICAL AND PHYSIOLOGICAL CARDIAC HYPERTROPHY: NOVEL THERAPEUTIC STRATEGIES TO TREAT HEART FAILURE
McMullen, Julie R / Jennings, Garry L | 2007
263
INVESTIGATION OF THE MICROCIRCULATION AND THE STATE OF OXIDATIVE STRESS IN THE RAT AFTER SCORPION ENVENOMATION
Sahnoun, Z / Chaker‐Krichen, S / Kassis, M / Hakim, A / Hammami, S / Ghozzi, H / Bouayed, N / Bellasfar, Z / Zeghal, KM / Rebai, T | 2007
269
SPATIAL PULMONARY FLOW DISTRIBUTION IN RABBIT ISOLATED LUNGS IS A POOR REPRESENTATION OF THE SITUATION IN VIVO*
Hübler, M / Heller, AR / Bleyl, JU / Rössel, T / Stehr, SN / Koch, T | 2007
274
EFFECT OF HIGH SALT INTAKE ON LOCAL RENIN–ANGIOTENSIN SYSTEM AND VENTRICULAR DYSFUNCTION FOLLOWING MYOCARDIAL INFARCTION IN RATS
De Resende, Micheline M / Mill, José G | 2007
280
INVOLVEMENT OF THE ENDOTHELIN ETB RECEPTOR IN GENDER DIFFERENCES IN DEOXYCORTICOSTERONE ACETATE‐SALT‐INDUCED HYPERTENSION
Kawanishi, Hideaki / Hasegawa, Youichi / Nakano, Daisuke / Ohkita, Mamoru / Takaoka, Masanori / Ohno, Yukihiro / Matsumura, Yasuo | 2007
286
IDENTIFICATION OF LIGANDS BINDING SPECIFICALLY TO INFLAMMATORY INTESTINAL MUCOSA USING PHAGE DISPLAY
Takagi, Tamaki / Arisawa, Tomiyasu / Yamamoto, Kazumasa / Hirata, Ichiro / Nakano, Hiroshi / Sawada, Makoto | 2007
290
PHARMACOKINETICS AND PHARMACODYNAMICS OF A NITRIC OXIDE‐RELEASING DERIVATIVE OF ENALAPRIL IN MALE BEAGLES
Okuyama, Cristina E / Mendes, Gustavo Duarte / Faro, Renato / Rezende, Vinicius M / Lagos, Rodolfo Monaco / Astigarraga, Rafael E B / Antunes, Edson / De Nucci, Gilberto | 2007
296
MORPHOLOGICAL AND FUNCTIONAL CHANGES IN P‐GLYCOPROTEIN DURING DEXAMETHASONE‐INDUCED HEPATOMEGALY
Micuda, Stanislav / Fuksa, Leos / Mundlova, Lucie / Osterreicher, Jan / Mokry, Jaroslav / Cermanova, Jolana / Brcakova, Eva / Staud, Frantisek / Pokorna, Pavla / Martinkova, Jirina | 2007
304
INHIBITION OF NAD(P)H OXIDASE REDUCES FIBRONECTIN EXPRESSION IN STROKE‐PRONE RENOVASCULAR HYPERTENSIVE RAT BRAIN
Cui, Chunmei / Chen, Alex F / Jiang, Zongpei / Wu, Qingqing / Lin, Jianwen / Wen, Hongmei / Zeng, Jinsheng | 2007
310
CALCIUM ANTAGONIST PROPERTY OF CPU228, A DOFETILIDE DERIVATIVE, CONTRIBUTES TO ITS LOW INCIDENCE OF TORSADES DE POINTES IN RABBITS
Huang, Zhi‐Jiang / Dai, De‐Zai / Li, Na / Na, Tao / Ji, Min / Dai, Yin | 2007
318
DOES NITRIC OXIDE MODULATE TRANSMITTER RELEASE AT THE MAMMALIAN NEUROMUSCULAR JUNCTION?
Nickels, Travis J / Reed, Grant W / Drummond, Justin T / Blevins, Dustin E / Lutz, Megan C / Wilson, David F | 2007
327
NITRIC OXIDE, ERECTILE DYSFUNCTION AND BETA‐BLOCKER TREATMENT (MR NOED STUDY): BENEFIT OF NEBIVOLOL VERSUS METOPROLOL IN HYPERTENSIVE MEN
Brixius, K / Middeke, M / Lichtenthal, A / Jahn, E / Schwinger, R H G | 2007
332
EFFECTS OF HYPOXIA/REPERFUSION INJURY ON DRUG DISPOSITION IN THE RAT ISOLATED PERFUSED LIVER
Arab, HA / Cheung, K / Hickman, PE / Potter, JM / Kadkhodaee, M / Roberts, MS | 2007
339
INHIBITION OF INDUCIBLE NITRIC OXIDE SYNTHASE ATTENUATES ACUTE ENDOTOXIN‐INDUCED LUNG INJURY IN RATS
Su, Chain Fa / Yang, Fwu Lin / Chen, Hsing I | 2007
347
ADMINISTRATION OF EXOGENOUS ERYTHROPOIETIN b AFFECTS LIPID PEROXIDATION AND SERUM PARAOXONASE-1 ACTIVITY AND CONCENTRATION IN PREDIALYSIS PATIENTS WITH CHRONIC RENAL DISEASE AND ANAEMIA:
Marsillach, J. / Martinez-Vea, A. / Marcas, L. / Mackness, B. / Mackness, M. / Ferre, N. / Joven, J. / Camps, J. | 2007
347
ADMINISTRATION OF EXOGENOUS ERYTHROPOIETIN β AFFECTS LIPID PEROXIDATION AND SERUM PARAOXONASE‐1 ACTIVITY AND CONCENTRATION IN PREDIALYSIS PATIENTS WITH CHRONIC RENAL DISEASE AND ANAEMIA
Marsillach, Judit / Martínez‐Vea, Albert / Marcas, Luis / Mackness, Bharti / Mackness, Michael / Ferré, Natàlia / Joven, Jorge / Camps, Jordi | 2007
350
METHODS OF ANALYSIS AND PHYSIOLOGICAL RELEVANCE OF RHYTHMS IN SYMPATHETIC NERVE DISCHARGE
Barman, Susan M / Kenney, Michael J | 2007
356
SYMPATHETIC RHYTHMS AND NERVOUS INTEGRATION
Gilbey, Michael P | 2007
362
IDENTIFICATION OF BLOOD PRESSURE CONTROL MECHANISMS BY POWER SPECTRAL ANALYSIS
Stauss, Harald M | 2007
369
DIFFERENTIAL EFFECTS OF VASOPRESSINERGIC AND OXYTOCINERGIC PRE‐AUTONOMIC NEURONS ON CIRCULATORY CONTROL: REFLEX MECHANISMS AND CHANGES DURING EXERCISE
Michelini, Lisete Compagno | 2007
377
EXERCISE TRAINING AND SYMPATHETIC NERVOUS SYSTEM ACTIVITY: EVIDENCE FOR PHYSICAL ACTIVITY DEPENDENT NEURAL PLASTICITY
Mueller, Patrick J | 2007
385
CORRIGENDUM
| 2007

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