NEUROPROTECTIVE EFFECTS OF DAURICINE AGAINST APOPTOSIS INDUCED BY TRANSIENT FOCAL CEREBRAL ISCHAEMIA IN RATS VIA A MITOCHONDRIAL PATHWAY (English)

Clinical and Experimental Pharmacology and Physiology

Previous experimental studies have shown that dauricine can protect the brain against ischaemic damage, but the underlying mechanisms remain unknown. In the present study, we examined whether dauricine inhibits neuronal apoptosis in the penumbra in a rat model of transient focal cerebral ischaemia.

Male Wistar rats underwent a 90 min temporary occlusion of the middle cerebral artery. Dauricine (21, 42 and 84 mg/kg) was administered by intragastric gavage twice a day for 3 days before ischaemia. Rats were killed and brain samples were collected 24 h after ischaemia. Histopathological outcome was evaluated by haematoxylin–eosin staining. Apoptotic changes were evaluated by terminal deoxyribonucleotidyl transferase‐mediated dUTP–digoxigenin nick end‐labelling (TUNEL) for DNA fragmentation. The mitochondrial pathway was explored using immunohistochemistry for cytochrome c release, caspase 9 and caspase 3 activation, as well as by reverse transcription–polymerase chain reaction for determination of caspase 9 and caspase 3 mRNA expression.

Cytochrome c release, activation of caspase 9 and caspase 3 and DNA fragmentation were detected 24 h after ischaemia. Dauricine (42 and 84 mg/kg) pretreatment improved histopathological recovery, diminished DNA fragmentation and reduced cytochrome c release and activation of caspase 9 and caspase 3 in the penumbra at 24 h.

These findings suggest that dauricine attenuates apoptosis in the penumbra after transient focal cerebral ischaemia. The infarct‐reducing effects of dauricine may be due, in part, to the inhibition of apoptotic cell death via a mitochondrial pathway in the penumbra.

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Table of contents – Volume 34, Issue 3

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135
CHOLESTEROL, STATINS AND CANCER
Brown, Andrew J | 2007
142
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148
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153
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161
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166
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Fantinelli, Juliana C / Mosca, Susana M | 2007
170
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177
NEUROPROTECTIVE EFFECTS OF DAURICINE AGAINST APOPTOSIS INDUCED BY TRANSIENT FOCAL CEREBRAL ISCHAEMIA IN RATS VIA A MITOCHONDRIAL PATHWAY
Li, Yan‐Hong / Gong, Pei‐Li | 2007
185
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191
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198
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205
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Castoldi, G / Di Gioia, CRT / Travaglini, C / Busca, G / Redaelli, S / Bombardi, C / Stella, A | 2007
210
STOBADINE PROTECTS RAT KIDNEY AGAINST ISCHAEMIA/REPERFUSION INJURY
Guz, Galip / Demirogullari, Billur / Ulusu, Nuray N / Dogu, Cihangir / Demirtola, Arzu / Kavutcu, Mustafa / Omeroglu, Suna / Stefek, Milan / Karasu, Cimen | 2007
217
POTENTIAL FOR ENDOTHELIN‐1‐MEDIATED IMPAIRMENT OF CONTRACTILE ACTIVITY IN HYPERTENSION
Kolka, Cathryn M / Rattigan, Stephen / Richards, Stephen M / Clark, Michael G | 2007
223
ROLE OF DORSAL HIPPOCAMPAL CANNABINOID RECEPTORS AND NITRIC OXIDE IN ANXIETY LIKE BEHAVIOURS IN RATS USING THE ELEVATED PLUS‐MAZE TEST
Roohbakhsh, Ali / Moghaddam, Akbar Hajizadeh / Massoudi, Roohollah / Zarrindast, Mohammad‐Reza | 2007
230
DIFFERENTIAL GROWTH INHIBITION AND INDUCTION OF APOPTOSIS BY GOSSYPOL BETWEEN HCT116 AND HCT116/BAX−/– COLORECTAL CANCER CELLS
Zhang, Manchao / Liu, Hongpeng / Tian, Zhenkun / Huang, Jenny / Remo, Mylene / Li, Q Quentin | 2007
238
BENEFICIAL EFFECT OF XANTHOANGELOL, A CHALCONE COMPOUND FROM ANGELICA KEISKEI, ON LIPID METABOLISM IN STROKE‐PRONE SPONTANEOUSLY HYPERTENSIVE RATS
Ogawa, Hiroshi / Okada, Yukiko / Kamisako, Toshinori / Baba, Kimiye | 2007
244
AMELIORATIVE EFFECT OF CHLOROPHYTUM BORIVILIANUM ROOT ON LIPID METABOLISM IN HYPERLIPAEMIC RATS
Visavadiya, NP / Narasimhacharya, AVRL | 2007
250
ACUTE CORONARY LIGATION IN THE DOG INDUCES TIME‐DEPENDENT TRANSITIONAL CHANGES IN MITOCHONDRIAL CRISTA IN THE NON‐ISCHAEMIC VENTRICULAR MYOCARDIUM
McLachlan, Craig Steven / Almsherqi, Zakaria A / Chua, Kerin Sze Yi / Liew, Yu Ying / Low, Chwee Wah / Deng, Yuru | 2007
254
LETTER TO THE EDITOR
Palomeque, Jesús CG / Ruiz, Hugo G | 2007

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