STOBADINE PROTECTS RAT KIDNEY AGAINST ISCHAEMIA/REPERFUSION INJURY (English)

Clinical and Experimental Pharmacology and Physiology

Ischaemia–reperfusion (I/R) injury, one of the main causes of acute renal failure, still needs satisfactory treatment for routine clinical application. Stobadine, a novel synthetic pyridoindole anti‐oxidant, has the ability to reduce tissue injury induced by mechanisms involving reactive oxygen species during I/R. The aim of the present study was to determine the effects of stobadine on renal I/R injury.

Forty male Wistar rats were randomly divided into four groups as follows: sham, I/R, stobadine treated and I/R + stobadine treated. Stobadine (2 mg/kg, i.v.) was given intravenously to two groups of rats. The stobadine‐treated group was treated with stobadine following sham operation before the abdominal wall was closed, whereas the I/R + stobadine group received stobadine at the beginning of reperfusion. Renal I/R was achieved by occluding the renal arteries bilaterally for 40 min, followed by 6 h reperfusion. Immediately thereafter, blood was drawn and tissue samples were harvested to assess: (i) serum levels of blood urea nitrogen and creatinine; (ii) serum and/or tissue levels of malondialdehyde (MDA), glutathione (GSH), glucose 6‐phosphate dehydrogenase (G‐6PD), 6‐phosphogluconate dehydrogenase (6‐PGD), glutathione reductase (GR) and glutathione peroxidase (GPx); (iii) renal morphology; and (iv) immunohistochemical staining for P‐selectin.

Stobadine was able to significantly attenuate the renal dysfunction as a result of renal I/R injury. Iscahemia–reperfusion resulted in a significant increase in serum and kidney MDA levels and a decrease in serum and kidney GSH. Stobadine treatment at the beginning of reperfusion attenuated both the increased MDA levels and decreased GSH secondary to I/R injury. In addition, the decreased G‐6PD activity observed after I/R was significantly attenuated by stobadine treatment. Stobadine did not alter 6‐PGD activity after I/R. Neither GR nor GPx activity was significantly changed in the I/R alone or the I/R + stobadine groups compared with the sham group. In addition, stobadine decreased the morphological deterioration and high P‐selectin immunoreactivity secondary to renal I/R injury.

A pyridoindole anti‐oxidant, stobadine exerts a renal protective effect in renal I/R injury, which is probably due to its radical‐scavenging and anti‐oxidant activities.

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Table of contents – Volume 34, Issue 3

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Tables of content are generated automatically and are based on records of articles contained that are available in the TIB-Portal index. Due to missing records of articles, the volume display may be incomplete, even though the whole journal is available at TIB.

135
CHOLESTEROL, STATINS AND CANCER
Brown, Andrew J | 2007
142
EFFICACY OF SARGASSUM POLYCYSTUM (PHAEOPHYCEAE) SULPHATED POLYSACCHARIDE AGAINST PARACETAMOL‐INDUCED DNA FRAGMENTATION AND MODULATION OF MEMBRANE‐BOUND PHOSPHATASES DURING TOXIC HEPATITIS
Raghavendran, HB / Sathivel, A / Yogeeta, RSSK / Devaki, T | 2007
148
FUNCTIONAL IMPAIRMENT OF CARDIAC TRANSIENT OUTWARD K+ CURRENT AS A RESULT OF ABNORMALLY ALTERED CELLULAR ENVIRONMENT
Du, Zhimin / Chaoqian, Xu / Shan, Hongli / Lu, Yanjie / Ren, Nanqi | 2007
153
ANGIOTENSIN II‐INDUCED GROWTH OF VASCULAR SMOOTH MUSCLE CELLS IS ASSOCIATED WITH MODULATION OF CELL SURFACE AREA AND PLATELET‐DERIVED GROWTH FACTOR RECEPTOR EXPRESSION
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161
CHANGES IN THE IN VITRO PHARMACODYNAMIC PROPERTIES OF METOPROLOL IN ATRIA ISOLATED FROM SPONTANEOUSLY HYPERTENSIVE RATS
Di Verniero, Carla / Höcht, Christian / Opezzo, Javier A W / Taira, Carlos A | 2007
166
CARDIOPROTECTIVE EFFECTS OF A NON‐ALCOHOLIC EXTRACT OF RED WINE DURING ISCHAEMIA AND REPERFUSION IN SPONTANEOUSLY HYPERTENSIVE RATS
Fantinelli, Juliana C / Mosca, Susana M | 2007
170
EFFECTS OF SHRIMP (MACROBRACIUM ROSENBERGII)‐DERIVED CHITOSAN ON PLASMA LIPID PROFILE AND LIVER LIPID PEROXIDE LEVELS IN NORMO‐ AND HYPERCHOLESTEROLAEMIC RATS
Hossain, Shahdat / Rahman, Azizur / Kabir, Yearul / Shams, Ali Ahmed / Afros, Fahmida / Hashimoto, Michio | 2007
177
NEUROPROTECTIVE EFFECTS OF DAURICINE AGAINST APOPTOSIS INDUCED BY TRANSIENT FOCAL CEREBRAL ISCHAEMIA IN RATS VIA A MITOCHONDRIAL PATHWAY
Li, Yan‐Hong / Gong, Pei‐Li | 2007
185
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Zhou, Dong / Yang, Peng‐Yuan / Zhou, Bin / Rui, Yao‐Cheng | 2007
191
DIFFERENTIAL DEVELOPMENT OF CARBACHOL‐INDUCED DESENSITIZATION IN RECEPTOR‐MEDIATED Ca2+ INFLUX AND Ca2+ RELEASE PATHWAYS IN SMOOTH MUSCLE OF GUINEA‐PIG TAENIA CAECI
Hishinuma, Shigeru / Matsumoto, Yukio / Sato, Ryo / Saito, Masaki | 2007
198
AMINO ACID AND FATTY ACID COMPOSITION OF AN AQUEOUS EXTRACT OF CHANNA STRIATUS (HARUAN) THAT EXHIBITS ANTINOCICEPTIVE ACTIVITY
Zakaria, ZA / Mat Jais, AM / Goh, YM / Sulaiman, MR / Somchit, MN | 2007
205
ANGIOTENSIN II INCREASES TISSUE‐SPECIFIC INHIBITOR OF METALLOPROTEINASE‐2 EXPRESSION IN RAT AORTIC SMOOTH MUSCLE CELLS IN VIVO: EVIDENCE OF A PRESSURE‐INDEPENDENT EFFECT
Castoldi, G / Di Gioia, CRT / Travaglini, C / Busca, G / Redaelli, S / Bombardi, C / Stella, A | 2007
210
STOBADINE PROTECTS RAT KIDNEY AGAINST ISCHAEMIA/REPERFUSION INJURY
Guz, Galip / Demirogullari, Billur / Ulusu, Nuray N / Dogu, Cihangir / Demirtola, Arzu / Kavutcu, Mustafa / Omeroglu, Suna / Stefek, Milan / Karasu, Cimen | 2007
217
POTENTIAL FOR ENDOTHELIN‐1‐MEDIATED IMPAIRMENT OF CONTRACTILE ACTIVITY IN HYPERTENSION
Kolka, Cathryn M / Rattigan, Stephen / Richards, Stephen M / Clark, Michael G | 2007
223
ROLE OF DORSAL HIPPOCAMPAL CANNABINOID RECEPTORS AND NITRIC OXIDE IN ANXIETY LIKE BEHAVIOURS IN RATS USING THE ELEVATED PLUS‐MAZE TEST
Roohbakhsh, Ali / Moghaddam, Akbar Hajizadeh / Massoudi, Roohollah / Zarrindast, Mohammad‐Reza | 2007
230
DIFFERENTIAL GROWTH INHIBITION AND INDUCTION OF APOPTOSIS BY GOSSYPOL BETWEEN HCT116 AND HCT116/BAX−/– COLORECTAL CANCER CELLS
Zhang, Manchao / Liu, Hongpeng / Tian, Zhenkun / Huang, Jenny / Remo, Mylene / Li, Q Quentin | 2007
238
BENEFICIAL EFFECT OF XANTHOANGELOL, A CHALCONE COMPOUND FROM ANGELICA KEISKEI, ON LIPID METABOLISM IN STROKE‐PRONE SPONTANEOUSLY HYPERTENSIVE RATS
Ogawa, Hiroshi / Okada, Yukiko / Kamisako, Toshinori / Baba, Kimiye | 2007
244
AMELIORATIVE EFFECT OF CHLOROPHYTUM BORIVILIANUM ROOT ON LIPID METABOLISM IN HYPERLIPAEMIC RATS
Visavadiya, NP / Narasimhacharya, AVRL | 2007
250
ACUTE CORONARY LIGATION IN THE DOG INDUCES TIME‐DEPENDENT TRANSITIONAL CHANGES IN MITOCHONDRIAL CRISTA IN THE NON‐ISCHAEMIC VENTRICULAR MYOCARDIUM
McLachlan, Craig Steven / Almsherqi, Zakaria A / Chua, Kerin Sze Yi / Liew, Yu Ying / Low, Chwee Wah / Deng, Yuru | 2007
254
LETTER TO THE EDITOR
Palomeque, Jesús CG / Ruiz, Hugo G | 2007

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