ENDOTOXIN‐INDUCED ACUTE LUNG INJURY AND ORGAN DYSFUNCTION ARE ATTENUATED BY PENTOBARBITAL ANAESTHESIA (English)

Clinical and Experimental Pharmacology and Physiology

Acute lung injury (ALI) as a result of sepsis is a major cause of mortality. Certain anaesthetic agents have been reported to suppress pro‐inflammatory cytokines and inducible nitric oxide (NO) synthase (iNOS) activities. We investigated the effects of pentobarbital on ALI and organ functions after the administration of endotoxin.

Intravenous (i.v.) pentobarbital (20 or 40 mg/kg) was administered 5 min after lipopolysaccharide (LPS; 10 or 30 mg/kg via i.v. infusion). To avoid hypoxia and/or hypercapnia following anaesthesia, we installed a special chamber connected to a rodent ventilator to provide ventilation with 95% oxygen content and 5% nitrogen. The animal was kept at eucapnic conditions (arterial Pco2 at an average of 38 ± 2 mmHg).

We monitored the arterial pressure (AP) and heart rate (HR). Acute lung injury was evaluated by lung weight changes, protein concentration in bronchoalveolar lavage, and Evans blue leakage. Plasma nitrate/nitrite, methyl guanidine and biochemical factors were determined. Pathological and immunofluorescent examinations were performed to observe the lung changes and to determine the activities of pro‐inflammatory cytokines, nitrotyrosine and iNOS.

Lipopolysaccharide caused dose‐dependent systemic hypotension with an increase in the extent of ALI. The lung pathology included oedema and inflammatory cell infiltration. Accompanying the ALI, LPS elevated plasma nitrate/nitrite, methyl guanidine, blood urea nitrogen, lactic dehydrogenase, creatinine phosphokinase, glutamic transaminase and amylase. The lung tissue content of tumour necrosis factor‐α, interleukin‐lβ, iNOS and nitrotyrosine was increased following LPS administration. These changes were abrogated by pentobarbital anaesthesia.

Our results indicated that pentobarbital anaesthesia significantly augmented the LPS‐induced systemic hypotension. However, it attenuated the LPS‐induced ALI and organ dysfunctions. This agent also improved the survival rate following LPS at high and low doses. This mechanism may be related to the inhibitory effects on the increases in the production or activity of NO, free radicals, pro‐inflammatory cytokines, nitrotyrosine and iNOS.

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Table of contents – Volume 34, Issue 5-6

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Tables of content are generated automatically and are based on records of articles contained that are available in the TIB-Portal index. Due to missing records of articles, the volume display may be incomplete, even though the whole journal is available at TIB.

387
MECHANISMS UNDERLYING VASORELAXANT ACTION OF ASTRAGALOSIDE IV IN ISOLATED RAT AORTIC RINGS
Zhang, Chuan / Wang, Xu‐Hui / Zhong, Mei‐Fang / Liu, Run‐Hui / Li, Hui‐Liang / Zhang, Wei‐Dong / Chen, Hong | 2007
393
PROTECTIVE ROLE OF A NOVEL ERYTHROCYTE‐DERIVED DEPRESSING FACTOR ON BLOOD VESSELS OF RENOVASCULAR HYPERTENSIVE RATS
Pang, Huan / Wen, Yun‐yi / Ma, Ning / Wang, Yu‐tang / Shi, Lei | 2007
399
THYMOQUINONE SUPPLEMENTATION PREVENTS THE DEVELOPMENT OF GENTAMICIN‐INDUCED ACUTE RENAL TOXICITY IN RATS
Sayed‐Ahmed, Mohamed M / Nagi, Mahmoud N | 2007
406
ESTABLISHMENT OF RAT PRECISION‐CUT FIBROTIC LIVER SLICE TECHNIQUE AND ITS APPLICATION IN VERAPAMIL METABOLISM
Guo, Yu / Wang, Hui / Zhang, Chun | 2007
414
STUDIES WITH KETAMINE AND ALFENTANIL FOLLOWING FREUND'S COMPLETE ADJUVANT‐INDUCED INFLAMMATION IN RATS
Edwards, Stephen R / Mather, Laurence E / Smith, Maree T | 2007
421
CYP2C19 GENOTYPE AND OMEPRAZOLE HYDROXYLATION PHENOTYPE IN CHINESE LI POPULATION
Wang, Jiu‐Hui / Li, Pei‐Qiong / Fu, Qiong‐Yao / Li, Qiu‐Xiang / Cai, Wang‐Wei | 2007
425
STRESS‐DEPENDENT INDUCTION OF PROTEIN OXIDATION, LIPID PEROXIDATION AND ANTI‐OXIDANTS IN PERIPHERAL TISSUES OF RATS: COMPARISON OF THREE STRESS MODELS (IMMOBILIZATION, COLD AND IMMOBILIZATION–COLD)
Şahın, Emel / Gümüşlü, Saadet | 2007
432
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Ren, Jun | 2007
439
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450
siRNA‐MEDIATED BCL‐2 AND BCL‐XL GENE SILENCING SENSITIZES HUMAN HEPATOBLASTOMA CELLS TO CHEMOTHERAPEUTIC DRUGS
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457
ROSIGLITAZONE, AN AGONIST OF PEROXISOME PROLIFERATOR‐ACTIVATED RECEPTOR‐GAMMA, PREVENTS CONTRALATERAL TESTICULAR ISCHAEMIA–REPERFUSION INJURY IN PREPUBERTAL RATS
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462
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Odagaki, Yuji / Toyoshima, Ryoichi | 2007
462
5-HT1A RECEPTOR AGONIST PROPERTIES OF ANTIPSYCHOTICS DETERMINED BY [35S]GTPgS BINDING IN RAT HIPPOCAMPAL MEMBRANES
Odagaki, Y. / Toyoshima, R. | 2007
467
PROTECTIVE EFFECTS OF VITAMINS C AND E AGAINST ENDOMETRIAL DAMAGE AND OXIDATIVE STRESS IN FLUORIDE INTOXICATION
Guney, Mehmet / Oral, Baha / Demirin, Hilmi / Karahan, Nermin / Mungan, Tamer / Delibas, Namik | 2007
475
LACK OF BLOOD PRESSURE SALT‐SENSITIVITY SUPPORTS A PREGLOMERULAR SITE OF ACTION OF NITRIC OXIDE IN TYPE I DIABETIC RATS
Brands, Michael W / Bell, Tracy D / Fleming, Cassandra / Labazi, Hicham / Sturgis, LaShon C | 2007
480
ENDOTOXIN‐INDUCED ACUTE LUNG INJURY AND ORGAN DYSFUNCTION ARE ATTENUATED BY PENTOBARBITAL ANAESTHESIA
Kao, Shang‐Jyh / Su, Chain‐Fa / Liu, Demeral D / Chen, Hsing I | 2007
488
THE EFFECTS OF ISOSTEVIOL AGAINST MYOCARDIUM INJURY INDUCED BY ISCHAEMIA–REPERFUSION IN THE ISOLATED GUINEA PIG HEART
Xu, Deyi / Zhang, Shuangjie / Foster, David JR / Wang, Jiping | 2007
494
DRUG‐RELATED HOSPITAL ADMISSIONS IN A GENERIC PHARMACEUTICAL SYSTEM
Zargarzadeh, AH / Emami, MH / Hosseini, F | 2007
499
OPTIMIZING DOSAGE OF KETAMINE AND XYLAZINE IN MURINE ECHOCARDIOGRAPHY
Xu, Qi / Ming, Ziqiu / Dart, Anthony M / Du, Xiao‐Jun | 2007
508
WRITING INTELLIGIBLE ENGLISH PROSE FOR BIOMEDICAL JOURNALS
Ludbrook, John | 2007
515
SLOW INOTROPIC RESPONSE OF INTACT LEFT VENTRICLE TO SUDDEN DILATION CRITICALLY DEPENDS ON A MYOCARDIAL DIALYSABLE FACTOR
Tucci, Paulo JF / Faber, Cristiano N / Santos, Leonardo dos / Antonio, Ednei L | 2007
517
ROLE OF XANTHINE OXIDASE IN DEXAMETHASONE‐INDUCED HYPERTENSION IN RATS
Ong, Sharon LH / Vickers, Janine J / Zhang, Yi / McKenzie, Katja US / Walsh, Claire E / Whitworth, Judith A | 2007
520
QUANTITATIVE CHARACTERIZATION OF A REPEATED ACUTE JOINT INFLAMMATION MODEL IN RATS
Peter‐Szabo, Mihaly / Kekesi, Gabriella / Nagy, Edit / Sziver, Edit / Benedek, Gyorgy / Horvath, Gyongyi | 2007
527
INTRODUCTION
Hannan, Anthony J | 2007
528
PROGENITOR CELLS AND ADULT NEUROGENESIS IN NEURODEGENERATIVE DISEASES AND INJURIES OF THE BASAL GANGLIA
Curtis, Maurice A / Eriksson, Peter S / Faull, Richard LM | 2007
533
REGULATORS OF ADULT NEUROGENESIS IN THE HEALTHY AND DISEASED BRAIN
Grote, Helen E / Hannan, Anthony J | 2007
546
5‐HT7, NEUROGENESIS AND ANTIDEPRESSANTS: A PROMISING THERAPEUTIC AXIS FOR TREATING DEPRESSION
Nandam, L Sanjay / Jhaveri, Dhanisha / Bartlett, Perry | 2007

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