SIMULTANEOUS GENOTYPING OF CYP2D6*3, *4, *5 AND *6 POLYMORPHISMS IN A SPANISH POPULATION THROUGH MULTIPLEX LONG POLYMERASE CHAIN REACTION AND MINISEQUENCING MULTIPLEX SINGLE BASE EXTENSION ANALYSIS (English)

Clinical and Experimental Pharmacology and Physiology

The aim of the present study was to perform a descriptive study of the prevalence of the four major CYP2D6 poor metaboliser (PM) alleles (*3, *4, *5 and *6) in a Spanish population (n = 290) using a method based on a new combination of multiplex long polymerase chain reaction (PCR) and minisequencing through multiplex single base extension (SBE) analysis.

The method was validated using different strategies, such as allelic discrimination assay and PCR–restriction fragment length polymorphism (RFLP).

The allele frequencies were similar to those described for other Spanish populations, namely 0.9% (95% confidence interval (CI) 0.5–1.3), 16.4% (95% CI 14.9–18.0), 2.7% (95% CI 2.0–3.4) and 0.7% (95% CI 0.3–1.0) for the *3, *4, *5 and *6 alleles, respectively. The results were satisfactory and left little doubt as to the genotypes, which were confirmed either by allelic discrimination assay (*4 and *6) or PCR‐RFLP (*3) with 100% concordance.

The present study corroborates the low prevalence of the most frequent polymorphism (CYP2D6*4) that leads to null CYP2D6 activity in Spain and the allelic geographical gradient between Caucasian populations in the north and south. The present study reports a technique for the detection of four polymorphisms that account for 98% of the CYP2D6 defect alleles. This multiplex long PCR–SBE technique is a combination of several known methods to genotype CYP2D6 alleles (*3, *4, *5 and*6). Given the importance of CYP2D6 in drug metabolism and the need to genotype a large number of samples, we believe that this method will find broad application.

How to get this document?
Download
Commercial Copyright fee: €37.76 Basic fee: €4.00 Total price: €41.76
Academic Copyright fee: €37.76 Basic fee: €2.00 Total price: €39.76

Document information


Table of contents – Volume 34, Issue 10

Show all volumes and issues

Tables of content are generated automatically and are based on records of articles contained that are available in the TIB-Portal index. Due to missing records of articles, the volume display may be incomplete, even though the whole journal is available at TIB.

955
SENSORY NEURAL TARGETS FOR THE TREATMENT OF COUGH
Mazzone, Stuart B / McGovern, Alice E | 2007
963
WHAT MAKES A CGRP2 RECEPTOR?
Hay, DL | 2007
972
17β‐OESTRADIOL PARTIALLY ATTENUATES THE INHIBITION OF NITRIC OXIDE SYNTHASE‐3 BY ADVANCED GLYCATION END‐PRODUCTS IN HUMAN PLATELETS
Chen, Lu / Liu, Yuan / Cui, Bota / Mi, Qiongyu / Huang, Yan / Fan, Leming / Chen, Qi / Tang, James / Ferro, Albert / Ji, Yong | 2007
972
17b-OESTRADIOL PARTIALLY ATTENUATES THE INHIBITION OF NITRIC OXIDE SYNTHASE-3 BY ADVANCED GLYCATION END-PRODUCTS IN HUMAN PLATELETS
Chen, L. / Liu, Y. / Cui, B. / Mi, Q. / Huang, Y. / Fan, L. / Chen, Q. / Tang, J. / Ferro, A. / Ji, Y. | 2007
979
VASODILATATION INDUCED BY SINOMENINE LOWERS BLOOD PRESSURE IN SPONTANEOUSLY HYPERTENSIVE RATS
Lee, Ping‐Ying / Chen, Wency / Liu, I‐Min / Cheng, Juei‐Tang | 2007
985
EFFECT OF ANGIOTENSIN AT1 RECEPTOR ANTAGONIST ON d‐GALACTOSAMINE‐INDUCED ACUTE LIVER INJURY
Chan, Herman / Leung, Po‐Sing / Tam, Michael S‐C | 2007
992
SIMULTANEOUS GENOTYPING OF CYP2D6*3, *4, *5 AND *6 POLYMORPHISMS IN A SPANISH POPULATION THROUGH MULTIPLEX LONG POLYMERASE CHAIN REACTION AND MINISEQUENCING MULTIPLEX SINGLE BASE EXTENSION ANALYSIS
Crescenti, A / Mas, S / Gassó, P / Baiget, M / Bernardo, M / Lafuente, A | 2007
998
17β‐OESTRADIOL REGULATES THE EXPRESSION OF Na+/K+‐ATPase β1‐SUBUNIT, SARCOPLASMIC RETICULUM Ca2+‐ATPase AND CARBONIC ANHYDRASE IV IN H9C2 CELLS
Liu, Chen‐Geng / Xu, Ke‐Qian / Xu, Xia / Huang, Jian‐Jun / Xiao, Jing‐Chuan / Zhang, Jie‐Ping / Song, Hui‐Ping | 2007
998
17b-OESTRADIOL REGULATES THE EXPRESSION OF Na+/K+-ATPase b1-SUBUNIT, SARCOPLASMIC RETICULUM Ca2+-ATPase AND CARBONIC ANHYDRASE IV IN H9C2 CELLS
Liu, C. G. / Xu, K. Q. / Xu, X. / Huang, J. J. / Xiao, J. C. / Zhang, J. P. / Song, H. P. | 2007
1005
INDICES OF VASCULAR STIFFNESS AND WAVE REFLECTION IN RELATION TO BODY MASS INDEX OR BODY FAT IN HEALTHY SUBJECTS
Wykretowicz, Andrzej / Adamska, Karolina / Guzik, Przemyslaw / Krauze, Tomasz / Wysocki, Henryk | 2007
1010
ANTICONVULSANT, BUT NOT ANTIEPILEPTIC, ACTION OF VALPROATE ON AUDIOGENIC SEIZURES IN METAPHIT–TREATED RATS
Stanojlović, Olivera P / Hrnčić, Dragan R / Živanović, Dragana P / Šušić, Veselinka T | 2007
1016
ROLE OF MACROPHAGES IN COMPLICATIONS OF TYPE 2 DIABETES
Tesch, GH | 2007
1020
HUMAN HEART β‐ADRENOCEPTORS: β1‐ADRENOCEPTOR DIVERSIFICATION THROUGH ‘AFFINITY STATES’ AND POLYMORPHISM
Molenaar, P / Chen, L / Semmler, ABT / Parsonage, WA / Kaumann, AJ | 2007
1020
HUMAN HEART b-ADRENOCEPTORS: b1-ADRENOCEPTOR DIVERSIFICATION THROUGH `AFFINITY STATES' AND POLYMORPHISM
Molenaar, P. / Chen, L. / Semmler, A. / Parsonage, W. / Kaumann, A. | 2007
1029
β2‐ADRENOCEPTOR POLYMORPHISMS AND OBSTRUCTIVE AIRWAY DISEASES: IMPORTANT ISSUES OF STUDY DESIGN
Yang, Ian A / Ng, Taria / Molenaar, Peter / Fong, Kwun M | 2007
1029
b2-ADRENOCEPTOR POLYMORPHISMS AND OBSTRUCTIVE AIRWAY DISEASES: IMPORTANT ISSUES OF STUDY DESIGN
Yang, I. A. / Ng, T. / Molenaar, P. / Fong, K. M. | 2007
1037
EFFECT OF GENDER AND SEX HORMONES ON VASCULAR OXIDATIVE STRESS
Miller, Alyson A / De Silva, T Michael / Jackman, Katherine A / Sobey, Christopher G | 2007
1044
COMBUSTION‐DERIVED NANOPARTICLES: MECHANISMS OF PULMONARY TOXICITY
BéruBé, Kelly / Balharry, Dominique / Sexton, Keith / Koshy, Lata / Jones, Tim | 2007
1051
POTENTIATION OF 3,4‐METHYLENEDIOXYMETHAMPHETAMINE‐INDUCED 5‐HT RELEASE IN THE RAT SUBSTANTIA NIGRA BY CLORGYLINE, A MONOAMINE OXIDASE A INHIBITOR
Hewton, Ryan / Salem, Abdallah / Irvine, Rodney J | 2007
1058
GLUTAMATE RECEPTOR PLASTICITY AT EXCITATORY SYNAPSES IN THE BRAIN
Genoux, David / Montgomery, Johanna M | 2007
1064
INHIBITORY INTERNEURONS IN THE PIRIFORM CORTEX
Suzuki, Norimitsu / Bekkers, John M | 2007
1070
DOES SPIKE TIMING‐DEPENDENT SYNAPTIC PLASTICITY UNDERLIE MEMORY FORMATION?
Letzkus, Johannes J / Kampa, Björn M / Stuart, Greg J | 2007
1077
FUNCTIONS OF SK CHANNELS IN CENTRAL NEURONS
Faber, ES Louise / Sah, Pankaj | 2007
1084
INTRACELLULAR CALCIUM IN THE FERTILIZATION AND DEVELOPMENT OF MAMMALIAN EGGS
Jones, Keith T | 2007

Similar titles