17β‐OESTRADIOL REGULATES THE EXPRESSION OF Na+/K+‐ATPase β1‐SUBUNIT, SARCOPLASMIC RETICULUM Ca2+‐ATPase AND CARBONIC ANHYDRASE IV IN H9C2 CELLS (English)

Clinical and Experimental Pharmacology and Physiology

It is necessary to improve our understanding of the effect of 17β‐oestradiol (E2) on the heart at a molecular and cellular level. In the present study, the effects of E2 on Na+/K+‐ATPase, sarcoplasmic/endoplasmic reticulum Ca2+‐ATPase (SERCA) and carbonic anhydrase IV (CAIV) in H9C2 cells were investigated. To identify the mechanism of action of E2 on these proteins, the oestrogen receptor (ER) antagonist tamoxifen was used.

The results indicated that 1 and 100 nmol/L E2 can enhance the activity of Na+/K+‐ATPase and SERCA and upregulate the expression of the Na+/K+‐ATPase β1‐subunit, SERCA2a and CAIV at both the mRNA and protein level compared with 0 and 0.01 nmol/L E2. 17β‐Oestradiol had the greatest effect at 100 nmol/L; 1 µmol/L E2 did not further protein expression compared with 100 nmol/L E2.

Tamoxifen (10 nmol/L) significantly decreased the activity of SERCA, as well as the expression of the Na+/K+‐ATPase β1‐subunit and SERCA at the mRNA and protein level, in H9C2 cells cultured with 1 nmol/L E2. Tamoxifen alone had no significant effect on these proteins in H9C2 cells.

It may be hypothesized that a suitable E2 concentration has a protective effect on the heart and that the actual dose of E2 used in hormone‐replacement therapy is important in menopausal women.

How to get this document?
Download
Commercial Copyright fee: €37.76 Basic fee: €4.00 Total price: €41.76
Academic Copyright fee: €37.76 Basic fee: €2.00 Total price: €39.76

Document information


Table of contents – Volume 34, Issue 10

Show all volumes and issues

Tables of content are generated automatically and are based on records of articles contained that are available in the TIB-Portal index. Due to missing records of articles, the volume display may be incomplete, even though the whole journal is available at TIB.

955
SENSORY NEURAL TARGETS FOR THE TREATMENT OF COUGH
Mazzone, Stuart B / McGovern, Alice E | 2007
963
WHAT MAKES A CGRP2 RECEPTOR?
Hay, DL | 2007
972
17b-OESTRADIOL PARTIALLY ATTENUATES THE INHIBITION OF NITRIC OXIDE SYNTHASE-3 BY ADVANCED GLYCATION END-PRODUCTS IN HUMAN PLATELETS
Chen, L. / Liu, Y. / Cui, B. / Mi, Q. / Huang, Y. / Fan, L. / Chen, Q. / Tang, J. / Ferro, A. / Ji, Y. | 2007
972
17β‐OESTRADIOL PARTIALLY ATTENUATES THE INHIBITION OF NITRIC OXIDE SYNTHASE‐3 BY ADVANCED GLYCATION END‐PRODUCTS IN HUMAN PLATELETS
Chen, Lu / Liu, Yuan / Cui, Bota / Mi, Qiongyu / Huang, Yan / Fan, Leming / Chen, Qi / Tang, James / Ferro, Albert / Ji, Yong | 2007
979
VASODILATATION INDUCED BY SINOMENINE LOWERS BLOOD PRESSURE IN SPONTANEOUSLY HYPERTENSIVE RATS
Lee, Ping‐Ying / Chen, Wency / Liu, I‐Min / Cheng, Juei‐Tang | 2007
985
EFFECT OF ANGIOTENSIN AT1 RECEPTOR ANTAGONIST ON d‐GALACTOSAMINE‐INDUCED ACUTE LIVER INJURY
Chan, Herman / Leung, Po‐Sing / Tam, Michael S‐C | 2007
992
SIMULTANEOUS GENOTYPING OF CYP2D6*3, *4, *5 AND *6 POLYMORPHISMS IN A SPANISH POPULATION THROUGH MULTIPLEX LONG POLYMERASE CHAIN REACTION AND MINISEQUENCING MULTIPLEX SINGLE BASE EXTENSION ANALYSIS
Crescenti, A / Mas, S / Gassó, P / Baiget, M / Bernardo, M / Lafuente, A | 2007
998
17b-OESTRADIOL REGULATES THE EXPRESSION OF Na+/K+-ATPase b1-SUBUNIT, SARCOPLASMIC RETICULUM Ca2+-ATPase AND CARBONIC ANHYDRASE IV IN H9C2 CELLS
Liu, C. G. / Xu, K. Q. / Xu, X. / Huang, J. J. / Xiao, J. C. / Zhang, J. P. / Song, H. P. | 2007
998
17β‐OESTRADIOL REGULATES THE EXPRESSION OF Na+/K+‐ATPase β1‐SUBUNIT, SARCOPLASMIC RETICULUM Ca2+‐ATPase AND CARBONIC ANHYDRASE IV IN H9C2 CELLS
Liu, Chen‐Geng / Xu, Ke‐Qian / Xu, Xia / Huang, Jian‐Jun / Xiao, Jing‐Chuan / Zhang, Jie‐Ping / Song, Hui‐Ping | 2007
1005
INDICES OF VASCULAR STIFFNESS AND WAVE REFLECTION IN RELATION TO BODY MASS INDEX OR BODY FAT IN HEALTHY SUBJECTS
Wykretowicz, Andrzej / Adamska, Karolina / Guzik, Przemyslaw / Krauze, Tomasz / Wysocki, Henryk | 2007
1010
ANTICONVULSANT, BUT NOT ANTIEPILEPTIC, ACTION OF VALPROATE ON AUDIOGENIC SEIZURES IN METAPHIT–TREATED RATS
Stanojlović, Olivera P / Hrnčić, Dragan R / Živanović, Dragana P / Šušić, Veselinka T | 2007
1016
ROLE OF MACROPHAGES IN COMPLICATIONS OF TYPE 2 DIABETES
Tesch, GH | 2007
1020
HUMAN HEART β‐ADRENOCEPTORS: β1‐ADRENOCEPTOR DIVERSIFICATION THROUGH ‘AFFINITY STATES’ AND POLYMORPHISM
Molenaar, P / Chen, L / Semmler, ABT / Parsonage, WA / Kaumann, AJ | 2007
1020
HUMAN HEART b-ADRENOCEPTORS: b1-ADRENOCEPTOR DIVERSIFICATION THROUGH `AFFINITY STATES' AND POLYMORPHISM
Molenaar, P. / Chen, L. / Semmler, A. / Parsonage, W. / Kaumann, A. | 2007
1029
b2-ADRENOCEPTOR POLYMORPHISMS AND OBSTRUCTIVE AIRWAY DISEASES: IMPORTANT ISSUES OF STUDY DESIGN
Yang, I. A. / Ng, T. / Molenaar, P. / Fong, K. M. | 2007
1029
β2‐ADRENOCEPTOR POLYMORPHISMS AND OBSTRUCTIVE AIRWAY DISEASES: IMPORTANT ISSUES OF STUDY DESIGN
Yang, Ian A / Ng, Taria / Molenaar, Peter / Fong, Kwun M | 2007
1037
EFFECT OF GENDER AND SEX HORMONES ON VASCULAR OXIDATIVE STRESS
Miller, Alyson A / De Silva, T Michael / Jackman, Katherine A / Sobey, Christopher G | 2007
1044
COMBUSTION‐DERIVED NANOPARTICLES: MECHANISMS OF PULMONARY TOXICITY
BéruBé, Kelly / Balharry, Dominique / Sexton, Keith / Koshy, Lata / Jones, Tim | 2007
1051
POTENTIATION OF 3,4‐METHYLENEDIOXYMETHAMPHETAMINE‐INDUCED 5‐HT RELEASE IN THE RAT SUBSTANTIA NIGRA BY CLORGYLINE, A MONOAMINE OXIDASE A INHIBITOR
Hewton, Ryan / Salem, Abdallah / Irvine, Rodney J | 2007
1058
GLUTAMATE RECEPTOR PLASTICITY AT EXCITATORY SYNAPSES IN THE BRAIN
Genoux, David / Montgomery, Johanna M | 2007
1064
INHIBITORY INTERNEURONS IN THE PIRIFORM CORTEX
Suzuki, Norimitsu / Bekkers, John M | 2007
1070
DOES SPIKE TIMING‐DEPENDENT SYNAPTIC PLASTICITY UNDERLIE MEMORY FORMATION?
Letzkus, Johannes J / Kampa, Björn M / Stuart, Greg J | 2007
1077
FUNCTIONS OF SK CHANNELS IN CENTRAL NEURONS
Faber, ES Louise / Sah, Pankaj | 2007
1084
INTRACELLULAR CALCIUM IN THE FERTILIZATION AND DEVELOPMENT OF MAMMALIAN EGGS
Jones, Keith T | 2007

Similar titles