17β‐OESTRADIOL PARTIALLY ATTENUATES THE INHIBITION OF NITRIC OXIDE SYNTHASE‐3 BY ADVANCED GLYCATION END‐PRODUCTS IN HUMAN PLATELETS (English)

Clinical and Experimental Pharmacology and Physiology

Diabetes mellitus predisposes to and female sex protects against arterial thrombosis. The aim of the present study was to determine whether advanced glycation end‐products (AGE), which accumulate in diabetes, impair platelet function through effects on platelet nitric oxide (NO) generation and whether this can be prevented by 17β‐oestradiol.

Aggregation responses of human platelet‐rich plasma to ADP were determined in the absence or presence of 200 mg/L AGE‐modified albumin (AGE‐albumin), 10−5 mol/L 17β‐oestradiol and 10−5 mol/L ICI 182 780 (the pure oestrogen receptor antagonist).

Intraplatelet cGMP, an index of bioactive NO, was measured by radioimmunoassay and expression of nitric oxide synthase (NOS)‐3, phosphoserine‐1177‐NOS‐3 and O‐glycosylated NOS‐3 was quantified by western blotting in response to these same treatments.

Advanced glycation end‐products–albumin increased platelet aggregatory responses to ADP. This increase was largely prevented by 17β‐oestradiol. Advanced glycation end‐products–albumin decreased and 17β‐oestradiol increased intraplatelet NO‐attributable cGMP and 17β‐oestradiol attenuated the AGE‐albumin‐induced decrease in NO‐attributable cGMP. Despite no effect on NOS‐3 expression, AGE‐albumin decreased and 17β‐oestradiol increased phosphoserine‐1177‐NOS‐3 and 17β‐oestradiol largely prevented the decrease in phosphoserine‐1177‐NOS‐3 induced by AGE‐albumin. Alone, AGE‐albumin increased O‐glycosylation of NOS‐3 by N‐acetylglucosamine, an effect largely inhibited by 17β‐oestradiol.

In conclusion, AGE‐albumin inhibits platelet NO biosynthesis through effects on serine phosphorylation andO‐glycosylation of platelet NOS‐3 and this may explain, at least in part, the increase in platelet aggregability induced by AGE‐albumin. These effects of AGE‐albumin are largely prevented by 17β‐oestradiol. These actions may contribute to the effects of diabetes and sex on arterial thrombosis in vivo.

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Table of contents – Volume 34, Issue 10

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Tables of content are generated automatically and are based on records of articles contained that are available in the TIB-Portal index. Due to missing records of articles, the volume display may be incomplete, even though the whole journal is available at TIB.

955
SENSORY NEURAL TARGETS FOR THE TREATMENT OF COUGH
Mazzone, Stuart B / McGovern, Alice E | 2007
963
WHAT MAKES A CGRP2 RECEPTOR?
Hay, DL | 2007
972
17β‐OESTRADIOL PARTIALLY ATTENUATES THE INHIBITION OF NITRIC OXIDE SYNTHASE‐3 BY ADVANCED GLYCATION END‐PRODUCTS IN HUMAN PLATELETS
Chen, Lu / Liu, Yuan / Cui, Bota / Mi, Qiongyu / Huang, Yan / Fan, Leming / Chen, Qi / Tang, James / Ferro, Albert / Ji, Yong | 2007
972
17b-OESTRADIOL PARTIALLY ATTENUATES THE INHIBITION OF NITRIC OXIDE SYNTHASE-3 BY ADVANCED GLYCATION END-PRODUCTS IN HUMAN PLATELETS
Chen, L. / Liu, Y. / Cui, B. / Mi, Q. / Huang, Y. / Fan, L. / Chen, Q. / Tang, J. / Ferro, A. / Ji, Y. | 2007
979
VASODILATATION INDUCED BY SINOMENINE LOWERS BLOOD PRESSURE IN SPONTANEOUSLY HYPERTENSIVE RATS
Lee, Ping‐Ying / Chen, Wency / Liu, I‐Min / Cheng, Juei‐Tang | 2007
985
EFFECT OF ANGIOTENSIN AT1 RECEPTOR ANTAGONIST ON d‐GALACTOSAMINE‐INDUCED ACUTE LIVER INJURY
Chan, Herman / Leung, Po‐Sing / Tam, Michael S‐C | 2007
992
SIMULTANEOUS GENOTYPING OF CYP2D6*3, *4, *5 AND *6 POLYMORPHISMS IN A SPANISH POPULATION THROUGH MULTIPLEX LONG POLYMERASE CHAIN REACTION AND MINISEQUENCING MULTIPLEX SINGLE BASE EXTENSION ANALYSIS
Crescenti, A / Mas, S / Gassó, P / Baiget, M / Bernardo, M / Lafuente, A | 2007
998
17β‐OESTRADIOL REGULATES THE EXPRESSION OF Na+/K+‐ATPase β1‐SUBUNIT, SARCOPLASMIC RETICULUM Ca2+‐ATPase AND CARBONIC ANHYDRASE IV IN H9C2 CELLS
Liu, Chen‐Geng / Xu, Ke‐Qian / Xu, Xia / Huang, Jian‐Jun / Xiao, Jing‐Chuan / Zhang, Jie‐Ping / Song, Hui‐Ping | 2007
998
17b-OESTRADIOL REGULATES THE EXPRESSION OF Na+/K+-ATPase b1-SUBUNIT, SARCOPLASMIC RETICULUM Ca2+-ATPase AND CARBONIC ANHYDRASE IV IN H9C2 CELLS
Liu, C. G. / Xu, K. Q. / Xu, X. / Huang, J. J. / Xiao, J. C. / Zhang, J. P. / Song, H. P. | 2007
1005
INDICES OF VASCULAR STIFFNESS AND WAVE REFLECTION IN RELATION TO BODY MASS INDEX OR BODY FAT IN HEALTHY SUBJECTS
Wykretowicz, Andrzej / Adamska, Karolina / Guzik, Przemyslaw / Krauze, Tomasz / Wysocki, Henryk | 2007
1010
ANTICONVULSANT, BUT NOT ANTIEPILEPTIC, ACTION OF VALPROATE ON AUDIOGENIC SEIZURES IN METAPHIT–TREATED RATS
Stanojlović, Olivera P / Hrnčić, Dragan R / Živanović, Dragana P / Šušić, Veselinka T | 2007
1016
ROLE OF MACROPHAGES IN COMPLICATIONS OF TYPE 2 DIABETES
Tesch, GH | 2007
1020
HUMAN HEART β‐ADRENOCEPTORS: β1‐ADRENOCEPTOR DIVERSIFICATION THROUGH ‘AFFINITY STATES’ AND POLYMORPHISM
Molenaar, P / Chen, L / Semmler, ABT / Parsonage, WA / Kaumann, AJ | 2007
1020
HUMAN HEART b-ADRENOCEPTORS: b1-ADRENOCEPTOR DIVERSIFICATION THROUGH `AFFINITY STATES' AND POLYMORPHISM
Molenaar, P. / Chen, L. / Semmler, A. / Parsonage, W. / Kaumann, A. | 2007
1029
β2‐ADRENOCEPTOR POLYMORPHISMS AND OBSTRUCTIVE AIRWAY DISEASES: IMPORTANT ISSUES OF STUDY DESIGN
Yang, Ian A / Ng, Taria / Molenaar, Peter / Fong, Kwun M | 2007
1029
b2-ADRENOCEPTOR POLYMORPHISMS AND OBSTRUCTIVE AIRWAY DISEASES: IMPORTANT ISSUES OF STUDY DESIGN
Yang, I. A. / Ng, T. / Molenaar, P. / Fong, K. M. | 2007
1037
EFFECT OF GENDER AND SEX HORMONES ON VASCULAR OXIDATIVE STRESS
Miller, Alyson A / De Silva, T Michael / Jackman, Katherine A / Sobey, Christopher G | 2007
1044
COMBUSTION‐DERIVED NANOPARTICLES: MECHANISMS OF PULMONARY TOXICITY
BéruBé, Kelly / Balharry, Dominique / Sexton, Keith / Koshy, Lata / Jones, Tim | 2007
1051
POTENTIATION OF 3,4‐METHYLENEDIOXYMETHAMPHETAMINE‐INDUCED 5‐HT RELEASE IN THE RAT SUBSTANTIA NIGRA BY CLORGYLINE, A MONOAMINE OXIDASE A INHIBITOR
Hewton, Ryan / Salem, Abdallah / Irvine, Rodney J | 2007
1058
GLUTAMATE RECEPTOR PLASTICITY AT EXCITATORY SYNAPSES IN THE BRAIN
Genoux, David / Montgomery, Johanna M | 2007
1064
INHIBITORY INTERNEURONS IN THE PIRIFORM CORTEX
Suzuki, Norimitsu / Bekkers, John M | 2007
1070
DOES SPIKE TIMING‐DEPENDENT SYNAPTIC PLASTICITY UNDERLIE MEMORY FORMATION?
Letzkus, Johannes J / Kampa, Björn M / Stuart, Greg J | 2007
1077
FUNCTIONS OF SK CHANNELS IN CENTRAL NEURONS
Faber, ES Louise / Sah, Pankaj | 2007
1084
INTRACELLULAR CALCIUM IN THE FERTILIZATION AND DEVELOPMENT OF MAMMALIAN EGGS
Jones, Keith T | 2007

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