Invasiveness and Intracellular Growth of Multidrug‐Resistant Salmonella and Other Pathogens in Caco‐2 Cells (English)

in Journal of Food Science ; 72 , 2 ; M72-M78
Journal of Food Science

ABSTRACT:  The increase of multidrug‐resistant pathogens of human and animal origins is a major public health concern. For a better understanding of the health consequences of multidrug‐resistant bacteria transmitted from animal products to humans, the host interaction of zoonotic Salmonella isolates along with other pathogenic and commensal bacteria was evaluated using a human intestinal Caco‐2 cell system. Multidrug‐resistant S. Agona, S. Heidelberg, and S. Typhimurium possessed plasmid‐mediated class 1 integrons. The S. Typhimurium DT104 isolate from ground beef showed the well‐known genotypic and phenotypic resistance characteristics of the species, and contained the chromosomally located class 1 integron. Among the multidrug‐resistant Salmonella isolates, the S. Heidelberg 219 had the highest invasion number at 1.0 × 104 CFU/mL, followed by the S. Typhimurium DT104 isolate at 7.7 × 103 CFU/mL. Listeria monocytogenes was the best performer among the tested species in invading the Caco‐2 cell. Multidrug‐resistant opportunistic pathogens Klebsiella pneumoniae and Pseudomonas aeruginosa were also able to invade the cells. The invasion of S. Heidelberg 219, S. Typhimurium DT104, L. monocytogenes, K. pneumoniae, and P. aeruginosa into the Caco‐2 cells was not affected even in the presence of commensal E. coli. During the intracellular growth of S. Heidelberg 219, S. Typhimurium DT104, and L. monocytogenes, the bacterial counts increased 2 log cycles in 9 h in the Caco‐2 cells. Therefore, these strains could rapidly proliferate after their invasion into the cells.

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Table of contents – Volume 72, Issue 2

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Tables of content are generated automatically and are based on records of articles contained that are available in the TIB-Portal index. Due to missing records of articles, the volume display may be incomplete, even though the whole journal is available at TIB.

C89
Effect of Ionic Strength and Temperature on Interaction between Fish Myoglobin and Myofibrillar Proteins
Chaijan, M. / Benjakul, S. / Visessanguan, W. / Lee, S. / Faustman, C. | 2007
C96
Preparation and Characterization of Protein Isolate from Fresh and Hardened Beans (Phaseolus vulgaris L.)
Morales‐de León, Josefina C. / Vázquez‐Mata, Norma / Torres, Nimbe / Gil‐Zenteno, Lidia / Bressani, Ricardo | 2007
C103
Characterization of Hydrolysates Derived from Enzymatic Hydrolysis of Wheat Gluten
Wang, Jin‐shui / Zhao, Mou‐ming / Zhao, Qiang‐zhong / Bao, Yang / Jiang, Yue‐ming | 2007
C108
Effect of Components Extracted from Okara on the Physicochemical Properties of Soymilk and Tofu Texture
Toda, Kyoko / Chiba, Kyoko / Ono, Tomotada | 2007
C114
Evaluation of Lipid Oxidation and Oxidative Products as Affected by Pork Meat Cut, Packaging Method, and Storage Time during Frozen Storage (−10 °C)
Park, S.Y. / Yoo, S.S. / Uh, J.H. / Eun, J.B. / Lee, H.C. / Kim, Y.J. / Chin, K.B. | 2007
C114
Evaluation of Lipid Oxidation and Oxidative Products as Affected by Pork Meat Cut, Packaging Method, and Storage Time during Frozen Storage (-10 degreeC):
Park, S. Y. / Yoo, S. S. / Uh, J. H. / Eun, J. B. / Lee, H. C. / Kim, Y. J. / Chin, K. B. | 2007
C120
Identification of C‐glycoside Flavonoids as Potential Mutagenic Compounds in Kava
Jhoo, J.‐W. / Ang, C. Y. W. / Heinze, T. M. / Deck, J. / Schnackenberg, L. K. / Beger, R. D. / Dragull, K. / Tang, C.‐S. | 2007
C126
Change in Mutagenicity in White Rice after Accelerated and Long‐Term Storage
Pahulu, H.F. / Davidson, R.T. / Dunn, M.L. / Ogden, L.V. / Steele, F.M. / Pike, O.A. | 2007
C132
Effects of Phosphorus Contents on the Gelatinization and Retrogradation of Potato Starch
Karim, A.A. / Toon, L.C. / Lee, V.P.L. / Ong, W.Y. / Fazilah, A. / Noda, T. | 2007
C139
Effect of Washing on Pesticide Residues in Olives
Guardia‐Rubio, M. / Ayora‐Cañada, M.J. / Ruiz‐Medina, A. | 2007
E49
Evaluation of Thermal Properties of Food Materials at High Pressures Using a Dual‐Needle Line‐Heat‐Source Method
Zhu, S. / Ramaswamy, H. S. / Marcotte, M. / Chen, C. / Shao, Y. / Le Bail, A. | 2007
E57
Microwave Heating of Cooked Pork Patties as a Function of Fat Content
Picouet, P.A. / Fernández, A. / Serra, X. / Suñol, J.J. / Arnau, J. | 2007
E64
Control of Lightness and Firmness of Cold and Reheated Frankfurter‐Type Sausages Using Different Spectroscopic Methods Applied to Raw Batter
Egelandsdal, B. / Dingstad, G.I. / Tøgersen, G. / Hildrum, K.I. | 2007
E73
Effect of Pretreatment of Hydrostatic Pressure on Physicochemical Properties of Tilapia Muscle Protein Gels Induced by Setting
Hsu, K.‐C. / Jao, C.‐L. | 2007
E79
Effects of Size of Cellulose Granules on Dough Rheology, Microscopy, and Breadmaking Properties
Seguchi, M. / Tabara, A. / Fukawa, I. / Ono, H. / Kumashiro, C. / Yoshino, Y. / Kusunose, C. / Yamane, C. | 2007
E85
Changes in Apple Liquid Phase Concentration throughout Equilibrium in Osmotic Dehydration
Barat, J.M. / Barrera, C. / Frías, J.M. / Fito, P. | 2007
E94
Physical Study of Minced Fish Muscle with a White‐Grape By‐Product Added as an Ingredient
Sánchez‐Alonso, I. / Solas, M.T. / Borderías, A.J. | 2007
E102
Modeling Texture Kinetics during Thermal Processing of Potato Products
Moyano, P.C. / Troncoso, E. / Pedreschi, F. | 2007
iv
Industrial Applications of Selected JFS Articles
| 2007
M39
Conversion of Isoflavone Glucosides to Aglycones in Soymilk by Fermentation with Lactic Acid Bacteria
Chun, Jiyeon / Kim, Gyoung Min / Lee, Kang Wook / Choi, In Duck / Kwon, Gun‐Hee / Park, Jae‐Young / Jeong, Seon‐Ju / Kim, Jeong‐Sang / Kim, Jeong Hwan | 2007
M45
Effect of Protective Agents on the Viability of Geotrichum candidum during Freeze‐Drying and Storage
Hamoudi, Latifa / Goulet, Jacques / Ratti, Cristina | 2007
M50
Effects of Short‐Chain Nitrocompounds against Campylobacter jejuni and Campylobacter coli in vitro
Horrocks, S.M. / Jung, Y.S. / Huwe, J.K. / Harvey, R.B. / Ricke, S.C. / Carstens, G.E. / Callaway, T.R. / Anderson, R.C. / Ramlachan, N. / Nisbet, D.J. | 2007
M56
Thermal Inactivation of Escherichia coli O157:H7, Salmonella, and Listeria monocytogenes in Breaded Pork Patties
Osaili, T. M. / Griffis, C. L. / Martin, E. M. / Beard, B. L. / Keener, A. E. / Marcy, J. A. | 2007
M62
Inactivation of Escherichia coli on Almonds Using Nonthermal Plasma
Deng, Shaobo / Ruan, Roger / Mok, Chul Kyoon / Huang, Guangwei / Lin, Xiangyang / Chen, Paul | 2007
M67
Expression and Purification of Goat Lactoferrin from Pichia pastoris Expression System
Chen, Gen‐Hung / Yin, Li‐Jung / Chiang, I‐Hua / Jiang, Shann‐Tzong | 2007
M72
Invasiveness and Intracellular Growth of Multidrug‐Resistant Salmonella and Other Pathogens in Caco‐2 Cells
Kim, S.‐H. / Wei, C.‐I. | 2007
R11
The Rheology of Colloidal and Noncolloidal Food Dispersions
Genovese, D. B. / Lozano, J. E. / Rao, M. A. | 2007
R21
Food Microstructure Affects the Bioavailability of Several Nutrients
Parada, J. / Aguilera, J.M. | 2007
R33
The Chemistry and Physiology of Sour Taste—A Review
Da Conceicao Neta, Edith Ramos / Johanningsmeier, Suzanne D. / McFeeters, Roger F. | 2007
S87
Consumer Sensory Analysis of Organically and Conventionally Grown Vegetables
Zhao, Xin / Chambers, Edgar IV / Matta, Ziad / Loughin, Thomas M. / Carey, Edward E. | 2007
S92
Acceptance and Purchase Intent of US Consumers for Nonwheat Rice Butter Cakes
Sae‐Eaw, A. / Chompreeda, P. / Prinyawiwatkul, W. / Haruthaithanasan, V. / Suwonsichon, T. / Saidu, J.E. / Xu, Z. | 2007
S98
Antioxidant Activities of Different Colored Sweet Bell Peppers (Capsicum annuum L.)
Sun, T. / Xu, Z. / Wu, C.‐T. / Janes, M. / Prinyawiwatkul, W. / No, H. K. | 2007
S103
Quality Changes of Salmon (Oncorhynchus gorbuscha) Muscle during Thermal Processing
Kong, Fanbin / Tang, Juming / Rasco, Barbara / Crapo, Chuck / Smiley, Scott | 2007
S112
Sensory and Physiochemical Characteristics of Frankfurters Formulated with Potassium Lactate and Sodium Diacetate before and after Irradiation
Knight, T.D. / Miller, R. / Maxim, J. / Keeton, J.T. | 2007
S119
Improvement of Moistness and Texture of High Omega‐3 Fatty Acid Mackerel Nuggets by Inclusion of Moisture‐Releasing Ingredients
Lee, K.H. / Joaquin, H. / Lee, C.M. | 2007
S125
Characterization of Hydrophobic Flavor Release Profile in Oil‐in‐Water Emulsions
Giroux, H.J. / Perreault, V. / Britten, M. | 2007
S130
The Influence of Processing and Preservation on the Retention of Health‐Promoting Compounds in Broccoli
Galgano, F. / Favati, F. / Caruso, M. / Pietrafesa, A. / Natella, S. | 2007
S136
Texture Profile of Tofu as Affected by Instron Parameters and Sample Preparation, and Correlations of Instron Hardness and Springiness with Sensory Scores
Yuan, S. / Chang, S. K. C. | 2007
S146
Sanitation Procedure Affects Biochemical and Nutritional Changes of Shredded Carrots
Ruiz‐Cruz, Saúl / Islas‐Osuna, María A. / Sotelo‐Mundo, Rogerio R. / Vázquez‐Ortiz, Francisco / González‐Aguilar, Gustavo A. | 2007
S153
Improvement of the in vitro Digestible Iron and Zinc Content of Okra (Hibiscus esculentus L.) Sauce Widely Consumed in Sahelian Africa
Avallone, Sylvie / Bohuon, Philippe / Hemery, Youna / Treche, Serge | 2007
S159
A Comparative Study on Phenolic Profiles and Antioxidant Activities of Legumes as Affected by Extraction Solvents
Xu, B.J. / Chang, S.K.C. | 2007
S167
Comparative Analyses of Phenolic Composition, Antioxidant Capacity, and Color of Cool Season Legumes and Other Selected Food Legumes
Xu, B.J. / Yuan, S.H. / Chang, S.K.C. | 2007
vi
Corrections: Vol. 72, Nr. 2
| 2007

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