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The Loop Current and its associated eddy-shedding in the Gulf of Mexico are mediating the oceanic heat and salt flux from the Caribbean into the Atlantic Ocean via Yucatan Strait. Changes in Yucatan Strait surface and intermediate through flow over geological timescales in relation to sea level, through flow velocity, and atmospheric circulation are not well constrained to date. The main objective of the geological sediment sampling program was to establish spatially and temporally high-resolving reconstructions of the Late Pleistocene surface, subsurface and intermediate water variability, in relation to the Loop Current variations and related eddy shedding, Antarctic Intermediate Water migrations, and changes in the Atlantic Meridional Overturning Circulation. The multi-channel seismic program was designed to establish a sequence stratigraphic framework for current controlled sediment formations which reflect the onset and intensification of the Gulfstream as well as Mid-Pleistocene climate transition. We focused on the main inflow area of Caribbean waters into the Gulf of Mexico, namely Yucatan Strait, and adjacent areas influenced by the Loop Current, i.e., the northeastern slope of Campeche Bank.