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Fertilization with mineral nitrogen labelled with the 15 isotope permits, in the crop, the estimation of both: - the real utilization coefficient of N fertilizer, - the uptake of the available N issued from organic matter. In 1979, such an experiment was carried out on sugar beet in Beauce on a long term field experiment including 7 N.P.K. treatments with 4 repetitions. Each plot was divided into 2 parts to constitute a treatment N=0 for the estimation of the apparent utilization coefficient. In addition, 4 microplots (size: 8 m exp 2 ) received a labelled fertilizer for the measurement of the actual coefficients. The results obtained show the incoherence of the apparent coefficients, which are higher than 100%. The real coefficients are from 40 to 80%. Thus, the balance sheet for the input and output of mineral nitrogen is established precisely. The gross mineralization can be much more important than the net mineralization. (Atomindex citation 13:647734)