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A study was made to document and evaluate effective technologies for the control of potential health hazards at the Wishard Memorial Hospital (SIC-8062), Indianapolis, Indiana. An evaluation was made of ways to maintain negative pressure in acid fast bacilli (AFB) isolation rooms, to quantify the parameters associated with negative pressure isolation rooms, and to evaluate the effectiveness of those parameters. Three nursery isolation rooms, and ten general isolation rooms were surveyed. Ventilation parameters were also collected from a pentamidine (100334) treatment area. Air was exhausted at a constant volume from the general isolation rooms through 16 inch by 16 inch grills located on the walls, near floor level and additional grills located in the ceiling of the bathrooms. The pentamidine treatment area consisted of a three sided booth within a large room. The booth was furnished with a cushioned chair for the patient. Exhaust was provided for the booth from a hood which enclosed the entire top of the booth. The nursery isolation rooms were provided with 100% outside air, conditioned and filtered.