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This report by the U.S. Geological Survey (USGS) provides the results of a rapid assessment of biological carbon stocks and biological carbon sequestration capacity in the conterminous United States. The rapid assessment, which includes estimates of storage and capacity for lands managed by the U.S. Department of the Interior (DOI), was requested by the DOI to provide timely and objective scientific information in support of the DOIs stewardship of land and natural resources. Assessment of nationwide biological carbon storage and carbon sequestration capacity is needed to determine effective ways to help reduce rising carbon dioxide (CO2) levels in the atmosphere. This rapid assessment also points toward the need for more data and knowledge to inform management and policy decisions.