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In this second, international symposium, we discussed several alternative approaches to combat the imminent antibiotic-resistance crisis that has the potential to lead to a post-antibiotic era. The first symposium was held in Mexico City on September 24-25, 205 at the Chemistry Institute, National Autonomous University of Mexico and led to the discovery of cisplatin as an effective DNA-crosslinking agent that eradicates a wide range of persister cells including enterohemorrhagic E. coli, Staphylococcus aureus, and P. aeruginosa. At this conference, for the confirmed speakers, Dr. Toms will speak on Iron metabolism of Acinetobacter baumannii as a new target for antimicrobial drugs, Dr. Licona Limn will speak on Design of immunotherapies for combating bacterial opportunistic pathogens, Dr. Garca Contreras will speak on Drug repurposing for treating A. baumannii and Pseudomonas aeruginosa infections, Dr. Martnez Vzquez will speak on Discovery of plant secondary metabolites as virulence inhibitors for P. aeruginosa and Chromatium violaceum, Dr. Wood will speak on New strategies for combating bacterial persister cells, Dr. Maeda will speak on Metabolism of quorum sensing inhibitors, Dr. Gonzlez Pedrajo will speak on Type III secretion systems in pathogenic bacteria, roles in virulence and as possible targets for anti-virulence therapies, Prof. Castillo Jurez will speak on in vitro and in vivo Quorum quenching and virulence inhibition, Prof. Sobern Chvez will speak on Dissecting the quorum sensing circuitry of Pseudomonas aeruginosa clinical strains for the design of robust antivirulence therapies, Dr. Hctor Quezada will speak on Applications of proteomics to the diagnosis and treatment of bacterial infections, Dr. Gonzlez Tinoco will speak on Inhibition of Pseudomonas aeruginosa virulence associated proteases. Therefore, the presentations will cover a wide range of topics related to antibiotic resistance and tolerance.