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A joint project was established to validate computational fluid dynamics (CFD) as a quantitative methodology for prediction of the distribution of pathogens released into the environmental control system (ECS)-generated ventilation flowfield of an aircraft passenger cabin. Acquisition of the requisite experimental databases for three dimensional velocity and contaminant distributions was accomplished in the FAA Civil Aerospace Medical Institute's (CAMI's) Aircraft Environmental Research Facility (AERF). The associated CFD simulations were conducted by the University of Tennessee CFD Laboratory staff, on the resident Beowulf PC cluster and/or the University of Tennessee Innovative Computing Laboratory SiNRG cluster, using both commercial and proprietary CFD computer codes. The results of this CFD validation project are reported herein.