Rechinoinsan Ni Yoru Shoki Genshi Senjoki Haiseigaihaiyo Karano Shimkei Saibo No Yudoteki Bunka (Inductive Differentiation of Nerve Cells in Mouse Embryonic Ectoderm at Early Primitive Streak Stage by Retinoic Acid)
(Englisch)
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Retinoic acid has a strong teratogenic effect, and it participates in embryonic development extensively. In previous studies, the mouse embryonic ectoderm was cultivated in vitro at the early primitive streak stage, and it was found that most embryos differentiated to epithelial cells and fibroblasts but did not seem to be committed to nerve cells. This study examined whether the embryonic ectoderm can differentiate to nerve cells in the presence of Retinoic Acid (RA). The embryos were extracted from mice at the early primitive streak stage, cultivated in a medium with RA for 20 hours, then removed to an RA-free medium. Cell differentiation was observed morphologically, and nerve cells were identified by the immunohistological method using anti-neurofilament monoclonal antibodies. As a result, the differentiation of nerve cells was observed at the fifth day of cultivation, and this was identified immunohistologically. However, the number of differentiated nerve cells was not constant among each culture.
Rechinoinsan Ni Yoru Shoki Genshi Senjoki Haiseigaihaiyo Karano Shimkei Saibo No Yudoteki Bunka (Inductive Differentiation of Nerve Cells in Mouse Embryonic Ectoderm at Early Primitive Streak Stage by Retinoic Acid)