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Electrohydrodynamics (EHD) micro-jet printing technology has become a new method of micro/nano patterning and fabrication because of high resolution, good compatibility and simple structure. Firstly, stresses that Taylor cone meniscus subjected to under pulse voltage are discussed in EHD micro-jet printing system and tangential electric stress grows faster than normal electric stress, and tangential electric stress helps to “push” jet generation. Secondly, apply a pulse voltage superimposed over DC voltage, and investigate correlations between pulse frequency and drop spacing and then pulse frequency and drop diameter, and then compare and analyze the theoretic results with the experimental ones. Finally, as a result that it’s possible to obtain droplets with almost the same interval and desired size in the certain situation through controlling pulse frequency.