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On the basis of previous work, this paper aims to build several proper drought indices based on the basic computation for the band information of passive microwave remote sensing AMSR-E data in Huaihe river basin. Compared with measured soil moisture, optimal drought indices have been selected to explore the spatio-temporal variation laws of drought conditions and its impact factors. The results indicate that there are satisfactory negative correlations between MPDIs (Microwave Polarization Difference Index) and observed soil moisture on the whole, which means the more negative the index is, the more serious the drought will be. Besides, MPDIs at frequency 69 GHz and 187 GHz calculated by AMSR-E brightness temperature data are much closer to the variation trend of soil moisture than those obtained from other bands.