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The aggregation phenomenon of hematoporphyrin and hematoporphyrin derivates in various solutions was investigated by FRAP (fluorescence recovery after photobleaching) technique. Nonlinear photodestruction quantum efficiency and diffusion coefficient of dye molecular complexes and proteins were measured. The method of laser microphotolysis, known as FRAP allows to develop adequate diagnostic methods and equipment for quantitative detection of structure and functional deflections in cells at molecular and submolecular levels. In the FRAP method the diffusion coefficient is determined by analysis of transient recovery of the fluorescence by diffusion of molecules into the illuminated spot following photobleaching of some of the fluorophore in this spot by a short intense laser pulse. The microphotolysis was based on a Nd-YAG-Laser, operating in the regime of microsecond pulse. Pulse production was carried out using a special optical modulator, enabling a power control and power control of pumping and bleaching laser pulses. Data processing and modulation control was carried out by computers.