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The term 'electroporation' refers to membrane pores caused by large transmembrane potentials. Some aspects of electroporation offer the possibility of applications in biology and medicine, with the introduction of DNA into cells representing the most prominent example. Prior studies of the transient high permeability state associated with electroporation are almost entirely based on the total response of a large population of cells. A method is described based on fluorescence-labeled test molecules and quantitative flow cytometer determinations using light scattering and fluorescence of individual cells in populations of 10000 or more cells. Computer-based calculations then allow analysis of subpopulation behavior.