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Per- and polyfluoroalkyl substances (PFAS) are a class of compounds with wide-ranging uses in industrial and commercial products and processes. Use of aqueous film-forming foams represents a known and/or potential source of PFAS contamination at many DoD sites due to frequent use of these foams during maintenance activities and fire training drills. The PFAS-containing aqueous film-forming foams were subsequently released directly to the ground or unlined pits. Additional sources of PFAS may also be present, as PFAS are used in a variety of applications (e.g., in certain paints, during chrome-plating, in photoprocessing) employed at DoD sites. The primary purpose of this evaluation is to develop risk-based screening levels (RBSL) protective of threatened and endangered species that are potentially present at DoD sites. Calculating RBSLs for aquatic life, terrestrial plants, soil invertebrates, and benthic invertebrates, requires acute and chronic toxicity data for individual PFAS. Calculating RBSLs for wildlife requires bioaccumulation factors and toxicity values for individual PFAS. Therefore, intermediate objectives of this evaluation include compiling appropriate toxicity and uptake data for individual PFAS and developing media and PFAS-specific bioaccumulation factors and toxicity values for wildlife.