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The project is designed to show that purified anti-cardiac myosin (or fragments thereof) will (1) localize in areas of myocardial damage; and (2) provide a useful tool for measuring the extent of such damage. Anti-myosin localization was enhanced by using (Fab1)2 fragments of anti-myosin - especially when (Fab1)2 uptake was determined at 72 hours after coronary occlusion in experimental animals. There is an inverse exponential relationship between anti-myosin (Fab1)2 localization and regional myocardial blood flow as determined by microsphere studies. Preliminary myocardial infarct Anger camera imaging was performed using Iodine-131 labelled anti-myosin (Fab1)2 fragments. 'Hot spot' images of myocardial infarction were superimposed on 'cold spot' images produced by radioactive microspheres in the same area of infarction. Thus, radioactive anti-myosin antibody fragments can be used to localize myocardial infarction in vivo by myocardial Anger camera imaging. Further refinements in techniques will hopefully allow imaging and 'sizing' of myocardial infarction in man.