A novel megaprimed and ligase-free, PCR-based, site-directed mutagenesis method (English)
- New search for: Tseng, Wen-Chi
- New search for: Lin, Jing-Wei
- New search for: Wei, Tsen-Yun
- New search for: Fang, Tsuei-Yun
- New search for: Tseng, Wen-Chi
- New search for: Lin, Jing-Wei
- New search for: Wei, Tsen-Yun
- New search for: Fang, Tsuei-Yun
In:
Analytical Biochemistry
;
375
, 2
;
376-378
;
2007
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ISSN:
- Article (Journal) / Electronic Resource
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Title:A novel megaprimed and ligase-free, PCR-based, site-directed mutagenesis method
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Contributors:Tseng, Wen-Chi ( author ) / Lin, Jing-Wei ( author ) / Wei, Tsen-Yun ( author ) / Fang, Tsuei-Yun ( author )
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Published in:Analytical Biochemistry ; 375, 2 ; 376-378
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Publisher:
- New search for: Elsevier Inc.
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Publication date:2007-11-02
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Size:3 pages
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ISSN:
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DOI:
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Type of media:Article (Journal)
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Type of material:Electronic Resource
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Language:English
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Source:
Table of contents – Volume 375, Issue 2
The tables of contents are generated automatically and are based on the data records of the individual contributions available in the index of the TIB portal. The display of the Tables of Contents may therefore be incomplete.
- 163
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Glycosylation profiling of a therapeutic recombinant monoclonal antibody with two N-linked glycosylation sites using liquid chromatography coupled to a hybrid quadrupole time-of-flight mass spectrometerLim, Amareth / Reed-Bogan, Angelia / Harmon, Bryan J. et al. | 2007
- 173
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TwinGFP, a marker for cell cycle analysis in transiently transfected cellsNiemantsverdriet, Maarten / Backendorf, Claude et al. | 2007
- 179
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An electrochemical approach for detection of specific DNA-binding protein by gold nanoparticle-catalyzed silver enhancementPan, Qin / Zhang, Renyun / Bai, Yunfei / He, Nongyue / Lu, Zuhong et al. | 2007
- 187
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Isotope-coded, iodoacetamide-based reagent to determine individual cysteine pKa values by matrix-assisted laser desorption/ionization time-of-flight mass spectrometryNelson, Kimberly J. / Day, Amanda E. / Zeng, Bu-Bing / King, S. Bruce / Poole, Leslie B. et al. | 2007
- 196
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Novel chromatographic resolution of chiral diacylglycerols and analysis of the stereoselective hydrolysis of triacylglycerols by lipasesRodriguez, J.A. / Mendoza, L.D. / Pezzotti, F. / Vanthuyne, N. / Leclaire, J. / Verger, R. / Buono, G. / Carriere, F. / Fotiadu, F. et al. | 2007
- 209
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Electrochemistry of cytochrome P450 enzyme on nanoparticle-containing membrane-coated electrode and its applications for drug sensingLiu, Songqin / Peng, Lei / Yang, Xiaodi / Wu, Yafeng / He, Lin et al. | 2007
- 217
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Rabbit antibody detection with RNA aptamersYoshida, Yoshihito / Sakai, Nobuya / Masuda, Hiromi / Furuichi, Makio / Nishikawa, Fumiko / Nishikawa, Satoshi / Mizuno, Hiroshi / Waga, Iwao et al. | 2007
- 223
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Evaluation of protein kinase activities of cell lysates using peptide microarrays based on surface plasmon resonance imagingMori, Takeshi / Inamori, Kazuki / Inoue, Yusuke / Han, Xiaoming / Yamanouchi, Go / Niidome, Takuro / Katayama, Yoshiki et al. | 2007
- 232
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One-pot fluorescent labeling of xyloglucan oligosaccharides with sulforhodamineKosík, Ondřej / Farkaš, Vladimír et al. | 2007
- 237
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A quantitative high-throughput screen identifies potential epigenetic modulators of gene expressionJohnson, Ronald L. / Huang, Wenwei / Jadhav, Ajit / Austin, Christopher P. / Inglese, James / Martinez, Elisabeth D. et al. | 2007
- 249
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A high-throughput assay for signal transducer and activator of transcription 5b based on fluorescence polarizationMüller, Judith / Schust, Jochen / Berg, Thorsten et al. | 2007
- 255
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Quantitative measurement of epidermal growth factor receptor–mitogen-activated protein kinase signal transduction using a nine-plex, peptide-based immunoassayRauh-Adelmann, Christine / Moskow, John M. / Graham, James R. / Yen, Lucy G. / Boucher, Jeffrey I. / Murphy, Cheryl E. / Nadler, Timothy K. / Gordon, Neal F. / Radding, Jeffrey A. et al. | 2007
- 265
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A fluorescent plate reader assay for ceramide kinaseDon, Anthony S. / Rosen, Hugh et al. | 2007
- 272
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Targeted metabolomic analysis of Escherichia coli by desorption electrospray ionization and extractive electrospray ionization mass spectrometryJackson, Ayanna U. / Werner, Sean R. / Talaty, Nari / Song, Yishu / Campbell, Karinna / Cooks, R. Graham / Morgan, John A. et al. | 2007
- 282
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Studies of the binding and signaling of surface-immobilized periplasmic glucose receptors on gold nanoparticles: A glucose biosensor applicationAndreescu, Silvana / Luck, Linda A. et al. | 2007
- 291
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High-performance liquid chromatographic assay with ultraviolet spectrometric detection for the evaluation of inhibitors of phosphatidylinositol-specific phospholipase CSchmolke, Marion / Fabian, Jörg / Lehr, Matthias et al. | 2007
- 299
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Detection of single-nucleotide polymorphisms using gold nanoparticles and single-strand-specific nucleasesChen, Yen-Ting / Hsu, Chiao-Ling / Hou, Shao-Yi et al. | 2007
- 306
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Development of sensitive cathepsin G fluorogenic substrate using combinatorial chemistry methodsLesner, Adam / Wysocka, Magdalena / Guzow, Katarzyna / Wiczk, Wiesław / Łęgowska, Anna / Rolka, Krzysztof et al. | 2007
- 313
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Protein screening using cold microwave technologySmith, Aaron G. / Johnson, Carol B. / Ann Ellis, E. / Vitha, Stanislav / Holzenburg, Andreas et al. | 2007
- 318
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FIT probes: Peptide nucleic acid probes with a fluorescent base surrogate enable real-time DNA quantification and single nucleotide polymorphism discoverySocher, Elke / Jarikote, Dilip V. / Knoll, Andrea / Röglin, Lars / Burmeister, Jens / Seitz, Oliver et al. | 2007
- 331
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Electrochemical studies of DNA immobilization onto the azide-terminated monolayers and its interaction with taxolMehdinia, Ali / Habib Kazemi, S. / Zahra Bathaie, S. / Alizadeh, Abdolhamid / Shamsipur, Mojtaba / Mousavi, Mir Fazlollah et al. | 2007
- 339
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Glutathione transferase activity with a novel substrate mimics the activation of the prodrug azathioprineKurtovic, Sanela / Grehn, Leif / Karlsson, Andreas / Hellman, Ulf / Mannervik, Bengt et al. | 2007
- 345
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Bienzyme amperometric biosensor using gold nanoparticle-modified electrodes for the determination of inulin in foodsManso, Javier / Mena, Ma Luz / Yáñez-Sedeño, P. / Pingarrón, José M. et al. | 2007
- 354
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Rapid quantification of global DNA methylation by isocratic cation exchange high-performance liquid chromatographyRozhon, Wilfried / Baubec, Tuncay / Mayerhofer, Juliane / Scheid, Ortrun Mittelsten / Jonak, Claudia et al. | 2007
- 361
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A method for the prevention of thrombin-induced degradation of recombinant proteinsRajalingam, Dakshinamurthy / Kathir, Karuppanan Muthusamy / Ananthamurthy, Koteshwara / Adams, Paul D. / Kumar, Thallapuranam Krishnaswamy Suresh et al. | 2007
- 364
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Highly sensitive assays for SUMOylation and small ubiquitin-like modifier-dependent protein–protein interactionsRouleau, Nathalie / Wang, Jianghai / Karras, Labrini / Andrews, Elizabeth / Bielefeld-Sevigny, Martina / Chen, Yuan et al. | 2007
- 367
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An HPLC-based fluorometric assay for serine hydroxymethyltransferaseZhang, Yi / Sun, Kehan / Roje, Sanja et al. | 2007
- 370
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Direct polymerase chain reaction (PCR) from human whole blood and filter-paper-dried blood by using a PCR buffer with a higher pHBu, Ying / Huang, Huan / Zhou, Guohua et al. | 2007
- 373
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PCR-mediated deletion of plasmid DNAHansson, Mattias D. / Rzeznicka, Kamila / Rosenbäck, Matilda / Hansson, Mats / Sirijovski, Nick et al. | 2007
- 376
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A novel megaprimed and ligase-free, PCR-based, site-directed mutagenesis methodTseng, Wen-Chi / Lin, Jing-Wei / Wei, Tsen-Yun / Fang, Tsuei-Yun et al. | 2007
- 379
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Concentration-independent estimation of protein secondary structure by circular dichroism: a comparison of methodsMcPhie, Peter et al. | 2007
- 382
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One- and two-dimensional SDS–PAGE zymography with quenched fluorogenic substrates provides identification of biological fluid proteases by direct mass spectrometryThimon, Véronique / Belghazi, Maya / Labas, Valérie / Dacheux, Jean-Louis / Gatti, Jean-Luc et al. | 2007
- 385
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An approach to assay calcineurin activity and the inhibitory effect of zinc ionHuang, Junyi / Zhang, Dongmei / Xing, Wei / Ma, Xiang / Yin, Yanxia / Wei, Qun / Li, Genxi et al. | 2007
- 388
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HIVcleave: a web-server for predicting human immunodeficiency virus protease cleavage sites in proteinsShen, Hong-Bin / Chou, Kuo-Chen et al. | 2007
- 391
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Enhanced solid phase PCR: mechanisms to increase priming by solid support primersKhan, Zaheer / Poetter, Karl / Park, Daniel J. et al. | 2007
- 394
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Intact recombineering of highly repetitive DNA requires reduced induction of recombination enzymes and improved host viabilityNarayanan, Kumaran et al. | 2007
- 397
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A nonradioisotope, enzymatic microplate assay for in vivo evaluation of 2-deoxyglucose uptake in muscle tissueYamamoto, Norio / Kawasaki, Kengo / Sato, Takuya / Hirose, Yoshitaka / Muroyama, Koutarou et al. | 2007
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Editorial Board| 2008