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Static ultrasound imaging based registration is an emerging navigation technique which requires a unique calibration matrix for each ultrasound probe. This is because of the fact that the ultrasound plane always has some rotation or translation with respect to the probes' housing. A calibration algorithm is developed to calculate such calibration matrix using a 3 N-thread phantom arrangement. In order to validate the calibration procedure the authors mathematically simulate various probes having some rotation or translation with respect to the probes' housing. This generated calibration matrix is stored in the EEPROM of the ultrasound probe. This calibrated navigated ultrasound system needs to be verified before it can be used. This paper describes a method to calibrate and verify the navigated ultrasound imaging system.