ANGIOTENSIN II RECEPTORS SUBTYPES MEDIATE DIVERSE GENE EXPRESSION PROFILE IN ADULT HYPERTROPHIC CARDIOMYOCYTES (English)

Clinical and Experimental Pharmacology and Physiology

Although the systemic and cardiac renin–angiotensin systems are known to be activated in the setting of pressure overload, the actions and signalling mechanisms of angiotensin (Ang) II via AT1 and AT2 receptors in hypertrophic cardiomyocytes (CM) remain largely unclear.

Hypertrophic CM were prepared from rats with aortic banding for 8 weeks, cultured and then treated as follows: (i) 1 µmol/L AngII for 24 h; (ii) 10 µmol/L losartan (an AT1 receptor antagonist) for 1 h followed by 1 µmol/L AngII for 24 h; and (iii) 10 µmol/L PD123319 (an AT2 receptor antagonist) for 1 h followed by 1 µmol/L AngII for 24 h. Changes in the expression of genes following stimulation of AT1 and AT2 receptors specific to G‐protein‐coupled receptor (GPCR) signalling pathways were tested using GEArray (Superarray, Bethesda, MD, USA). The effects of AngII, acting via AT1 and AT2 receptors, on the expression of tumour necrosis factor (TNF)‐α, interleukin (IL)‐1β and IL‐6 were confirmed by reverse transcription–polymerase chain reaction and radioimmunoassay.

The genes regulated via stimulation of AT1 receptors were mainly restricted to the signalling pathways including cAMP/protein kinase (PK) A, Ca2+, PKC, protein tyrosine kinase, mitogen‐activated protein kinases, phosphatidylinositol 3‐kinase and nuclear factor‐κB. In addition to these pathways related to activation of AT1 receptors, four additional signalling pathways were found to be associated with stimulation of AT2 receptors, including phospholipase C, nitric oxide/cGMP, Rho and Janus kinase/signal transducer and activator of transcription. Blockade of AT2 receptors decreased the mRNA and protein expression of TNF‐α and IL‐1β, whereas blockade of AT1 receptors had no such effect.

In conclusion, in hypertrophic CM, AngII leads to distinct signalling responses mediated by AT1 and AT2 receptors. Stimulation of AT2 receptors appears to have a greater influence on GPCR‐signalling than stimulation of AT1 receptors. Angiotensin II enhances the synthesis and secretion of TNF‐α and IL‐1β in hypertrophic CM, which is mediated by AT2, but not AT1, receptors.

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Table of contents – Volume 34, Issue 11

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Tables of content are generated automatically and are based on records of articles contained that are available in the TIB-Portal index. Due to missing records of articles, the volume display may be incomplete, even though the whole journal is available at TIB.

1091
AGE‐RELATED CHANGES IN THE STRUCTURE AND FUNCTION OF SKELETAL MUSCLES
Faulkner, John A / Larkin, Lisa M / Claflin, Dennis R / Brooks, Susan V | 2007
1097
MODULATION OF SIGNAL TRANSDUCERS AND ACTIVATORS OF TRANSCRIPTION (STAT) FACTOR PATHWAYS DURING FOCAL CEREBRAL ISCHAEMIA: A GENE EXPRESSION ARRAY STUDY IN RAT HIPPOCAMPUS AFTER MIDDLE CEREBRAL ARTERY OCCLUSION
Sun, Sheng‐Li / Li, Tie‐Jun / Yang, Peng‐Yuan / Qiu, Yan / Rui, Yao‐Cheng | 2007
1102
PROTEASOME INHIBITOR LACTACYSTIN ABLATES LIVER INJURY INDUCED BY INTESTINAL ISCHAEMIA–REPERFUSION
Yao, Ji‐Hong / Li, Ying‐Hua / Wang, Zhen‐Zhen / Zhang, Xue‐Song / Wang, Yu‐Zhu / Yuan, Jin‐Chan / Zhou, Qin / Liu, Ke‐Xin / Tian, Xiao‐Feng | 2007
1109
EFFECTS OF RESVERATROL ON NUMBER AND ACTIVITY OF ENDOTHELIAL PROGENITOR CELLS FROM HUMAN PERIPHERAL BLOOD
Wang, Xiao‐Bin / Huang, Jun / Zou, Jian‐Gang / Su, En‐Ben / Shan, Qi‐Jun / Yang, Zhi‐Jian / Cao, Ke‐Jiang | 2007
1116
FLAVONE INHIBITS VASCULAR CONTRACTION BY DECREASING PHOSPHORYLATION OF THE MYOSIN PHOSPHATASE TARGET SUBUNIT
Jeon, Su Bun / Kim, Gutae / Kim, Jee In / Seok, Young Mi / Kim, Sang‐Hyun / Suk, Kyoungho / Shin, Heung‐Mook / Lee, Young‐Ho / Kim, In Kyeom | 2007
1121
SPINAL α‐AMINO‐3‐HYDROXY‐5‐METHYL‐4‐ISOXAZOLEPROPIONIC ACID RECEPTORS MAY MEDIATE THE ANALGESIC EFFECTS OF EMULSIFIED HALOGENATED ANAESTHETICS
Hang, Li‐Hua / Shao, Dong‐Hua / Yang, Ying‐Hong / Dai, Ti‐Jun / Zeng, Yin‐Ming | 2007
1121
SPINAL a-AMINO-3-HYDROXY-5-METHYL-4-ISOXAZOLEPROPIONIC ACID RECEPTORS MAY MEDIATE THE ANALGESIC EFFECTS OF EMULSIFIED HALOGENATED ANAESTHETICS
Hang, L. H. / Shao, D. H. / Yang, Y. H. / Dai, T. J. / Zeng, Y. M. | 2007
1126
S100B LEVELS IN THE CEREBROSPINAL FLUID OF RATS ARE SEX AND ANAESTHETIC DEPENDENT
Vicente, Évelin / Tramontina, Francine / Leite, Marina C / Nardin, Patricia / Silva, Mariane / Karkow, Ana R / Adolf, Rafael / Lucion, Aldo B / Netto, Carlos A / Gottfried, Carmem et al. | 2007
1131
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1138
EXPRESSION OF CYP3A ISOFORMS AND P‐GLYCOPROTEIN IN HUMAN STOMACH, JEJUNUM AND ILEUM
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1145
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Hishinuma, Shigeru / Sato, Ryo / Saito, Masaki | 2007
1151
EFFECTS OF AGAR AND PECTIN ON GASTRIC EMPTYING AND POST‐PRANDIAL GLYCAEMIC PROFILES IN HEALTHY HUMAN VOLUNTEERS
Sanaka, Masaki / Yamamoto, Takatsugu / Anjiki, Hajime / Nagasawa, Kunitaka / Kuyama, Yasushi | 2007
1156
ACTIVATION OF PERIPHERAL CHEMORECEPTORS CAUSES POSITIVE INOTROPIC EFFECTS IN A WORKING HEART–BRAINSTEM PREPARATION OF THE RAT
Braga, Valdir A / Zoccal, Daniel B / Soriano, Renato N / Antunes, Vagner R / Paton, Julian F / Machado, Benedito H / Nalivaiko, Eugene | 2007
1160
RESTORATION OF ANTIBIOTIC SUSCEPTIBILITY IN METHICILLIN‐RESISTANT STAPHYLOCOCCUS AUREUS BY TARGETING MECR1 WITH A PHOSPHOROTHIOATE DEOXYRIBOZYME
Hou, Zheng / Meng, Jing‐Ru / Niu, Chao / Wang, Hai‐Fang / Liu, Jie / Hu, Ben‐Quan / Jia, Min / Luo, Xiao‐Xing | 2007
1165
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Guido, Maria C / De Carvalho Frimm, Clovis / Koike, Márcia K / Cordeiro, Fernanda F / Moretti, Ana IS / Godoy, Luiz C | 2007
1173
NITRIC OXIDE MODULATES AIR EMBOLISM‐INDUCED LUNG INJURY IN RATS WITH NORMOTENSION AND HYPERTENSION
Liu, Yen Chin / Kao, Shang Jyh / Chuang, I Chun / Chen, Hsing I | 2007
1181
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Cetin, Hasan / Olgar, şeref / Oktem, Faruk / Ciris, Metin / Uz, Efkan / Aslan, Çaǧatay / Ozguner, Fehmi | 2007
1186
ACTIONS OF THE ENDOCANNABINOID TRANSPORT INHIBITOR AM404 IN NEUROPATHIC AND INFLAMMATORY PAIN MODELS
Mitchell, Vanessa A / Greenwood, Ruth / Jayamanne, Angelo / Vaughan, Christopher W | 2007
1191
ANGIOTENSIN II RECEPTORS SUBTYPES MEDIATE DIVERSE GENE EXPRESSION PROFILE IN ADULT HYPERTROPHIC CARDIOMYOCYTES
Zhou, Juan / Xu, Xin / Liu, Jin‐Jun / Lin, Yuan‐Xi / Gao, Guang‐Dao | 2007
1199
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García, Mónica / Morán, Asunción / Martín, M Luisa / Ortizde Urbina, A Vega / San Román, Luis | 2007
1207
GENOTYPE AND ALLELE FREQUENCIES OF N‐ACETYLTRANSFERASE 2 AND GLUTATHIONE S‐TRANSFERASE IN THE IRANIAN POPULATION
Torkaman‐Boutorabi, Anahita / Hoormand, Mahmood / Naghdi, Naser / Bakhshayesh, Masoomeh / Milanian, Iraj | 2007
1212
EARLY RENAL DENERVATION PREVENTS DEVELOPMENT OF HYPERTENSION IN GROWTH‐RESTRICTED OFFSPRING
Ojeda, Norma B / Johnson, W Russell / Dwyer, Terry M / Alexander, Barbara T | 2007
1217
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